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- Human Iris Pigment Epithelial Cells
The iris is a pigmented disk with a variable aperture which controls the size of the pupil and the amount of light reaching the retina. It consists of the anterior limiting layer, the stroma, the dilator muscle layer, and the posterior pigmented epithelium. The iris pigment epithelial cells (IPEpiC) share the same embryonic origin as retinal pigment epithelial cells (RPE) and thus exhibit similar functional properties as RPE, including turnover of photoreceptors and synthesis of trophic factors [1]. Previous studies showed that IPEpiC transplanted into the subretinal space inhibited abnormal neovascularization and photoreceptor degeneration, which suggests that IPEpiC transplantation may be used in the future to treat retinal disorders [2].
HIPEpiC from ScienCell Research Laboratories are isolated from human iris. HIPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIPEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. HIPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HIPEpiC are guaranteed to further expand for 10 population doublings under the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for culturing HIPEpiC in vitro.
| Catalog No. | 6560 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HIPEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1]. Semkova, I., Kreppel, F., Welsandt, G., Luther, T., Kozlowski, J., Janicki, H., Kochanek, S. and Schraermeyer, U. (2004) Autologous transplantation of genetically modified iris pigment epithelial cells: a promising concept for the treatment of age-related macular degeneration and other disorders of the eye. PNAS 99(20):13090-13095. [2]. Crafoord, S. (2000) Experimental transplantation of retinal and iris pigment epithelial cells into the subretinal space. Student Journal of Health Sciences. 3(1):22. [3]. Sugita, S. and Streilein, J. W. (2004) Iris pigment epithelium expressing CD86 (B7-2) directly suppresses T cell activation in vitro via binding to cytotoxic T lymphocyte-associated antigen 4. J. Exp. Med. 198:161-171. |
1.) Chui JJ, Li MW, Di Girolamo N, Chang JH, McCluskey PJ, Wakefield D. (2010) "Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex." Invest Ophthalmol Vis Sci. 51: 2558-67.
2.) Chui JJ, Li MW, Di Girolamo N, Chang JH, McCluskey PJ, Wakefield D. (2010) "In vitro and in vivo characterization of iris pigment epithelial cells cultured on amniotic membranes." Mol Vis. 12: 1022-32.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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6560| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4101 | EpiCM | Epithelial Cell Medium |
$122.00
As low as:
$81.00
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| REAGENTS | |||||
| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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| 0923 | EpiF | EpiFectagen |
$253.00
|
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| MOLECULAR BIOLOGY | |||||
| 6564 | HIPEpiC cDNA | Human Iris Pigment Epithelial Cell cDNA |
$411.00
|
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| 6565 | HIPEpiC tRNA | Human Iris Pigment Epithelial Cell Total RNA |
$364.00
|
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| 6566 | HIPEpiC Lysate | Human Iris Pigment Epithelial Cell Lysate |
$398.00
|
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| 6567 | HIPEpiC miRNA | Human Iris Pigment Epithelial Cell MicroRNA |
$340.00
|
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| 6569 | HIPEpiC gDNA | Human Iris Pigment Epithelial Cell Genomic DNA |
$398.00
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