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- Human Mammary Fibroblasts
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. Mammary fibroblasts (MF) synthesize components of the stromal extracellular matrix of the mammary gland. More importantly, such stromal extracellular matrix regulates the proliferation and differentiation of mammary epithelial cells by influencing their gene expression. In addition, previous studies have shown that mammary fibroblasts play a role in recruiting macrophages for immune surveillance which, in concert with extracellular matrix turnover and angiogenesis, is associated with a supportive mammary microenvironment for tumor cell progression. These results suggest that MF play a stimulatory functional role in breast cancer development and invasion.
HMF from ScienCell Research Laboratories are isolated from human breast. HMF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMF are characterized by immunofluorescence with antibodies specific to fibronectin. HMF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HMF in vitro.
| Catalog No. | 7630 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HMF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] D Zangani, KM Darcy, PA Masso-Welch, ES Bellamy, MS Desole, MM Ip (1999) Multiple differentiation pathways of rat mammary stromal cells in vitro: acquisition of a fibroblast, adipocyte or endothelial phenotype is dependent on hormonal and extracellular matrix stimulation. Differentiation. 64(2)91-101. [2] WG Jiang, D Grimshaw, TA Martin, G Davies, C Parr, G Watkins, J Lane, R Abounader, J Laterra and RE Mansel (2003) Reduction of Stromal Fibroblast-induced Mammary Tumor Growth, by Retroviral Ribozyme Transgenes to Hepatocyte Growth Factor/Scatter Factor and its Receptor, c-MET. Clinical Cancer Research (9)4274-4281. |
76301.) Choi, Y., Hyun, E., Seo, J., Blundell, C., Kim, H.C., Lee, E., Lee, S., Moon, A., Moon, W.K. & Huh, D. (2015) A microengineered pathophysiological model of early-stage breast cancer Lab Chip. 15
2.) Dhimolea E, Maffini MV, Soto AM, Sonnenschein C. (2010) "The role of collagen reorganization on mammary epithelial morphogenesis in a 3D culture model." Biomaterials. 31: 3622-30.
3.) Krause S, Maffini MV, Soto AM, Sonnenschein C. (2010) "The microenvironment determines the breast cancer cells' phenotype: organization of MCF7 cells in 3D cultures." BMC Cancer. 10: 263.
4.) Wang X, Sun L, Maffini MV, Soto A, Sonnenschein C, Kaplan DL. (2010) "A complex 3D human tissue culture system based on mammary stromal cells and silk scaffolds for modeling breast morphogenesis and function." Biomaterials. 31: 3920-9.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 7634 | HMF cDNA | Human Mammary Fibroblast cDNA |
$453.00
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| 7635 | HMF tRNA | Human Mammary Fibroblast Total RNA |
$401.00
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| 7636 | HMF Lysate | Human Mammary Fibroblast Lysate |
$438.00
|
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| 7637 | HMF miRNA | Human Mammary Fibroblast MicroRNA |
$374.00
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| 7639 | HMF gDNA | Human Mammary Fibroblast Genomic DNA |
$438.00
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