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- Human Microglia
Microglia, one of the glial cell types in the central nervous system (CNS), is an important integral component of the neuro-glial cell network. They are present in the brain parenchyma from the early stage of development to the mature state. Microglia play an important role in brain immune surveillance. They can present antigens in the molecular context of MHC class II expression to CD-4 positive T cells, and are capable of Fc-mediated phagocytosis, and share many common antigens with hemopoietic and tissue macrophages. Upon activation, they act as brain macrophages to clear tissue debris, damaged cells, or microbes when programmed cell death occurs during brain development or when the CNS is injured. Furthermore, there is evidence that microglia are involved in a variety of physiological and pathological processes in the brain through interaction with neurons, other glial cells, and production of biologically active substances such as growth factors and cytokines.
HM from ScienCell Research Laboratories are isolated from human brain. HM are cryopreserved after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HM are characterized by immunofluorescence with antibodies to CD11b and/or F4/80. HM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HM are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.
Recommended Medium
It is recommended to use Microglia Medium (MM, Cat. #1901) for culturing HM in vitro.
| Catalog No. | 1900 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HM |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Transfer frozen cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Lee, S. C., Liu, W., Brosnan, C. F. and Dickson, D. W. (1992) Characterization of primary human fetal dissociated central nervous system cultures with an emphasis on microgia. Laboratory Investigation. 67:465-476. [2] Fedoroff, S., Zhai, R. and Novak, J. P. (1997) Microglia and astroglia have a common progenitor cell. J. Neurosci. Res. 50: 477-486. [3] Stoll, G. and Jander, S. (1999) The role of microglia and macrophages in the pathophysiology of the CNS. Prog. Neurobiol. 58:233-247. |
19001.) Zhou, C., Zhang, Y., Dai, J., Zhou, M., Liu, M., Wang, Y., Chen, X.Z. & Tang, J.(2016) 'Pygo2 functions as a prognostic factor for glioma due to its upregulation of H3K4me3 and promotion of MLL1/MLL2 complex recruitment' Sci Rep. VOL 6
2.) El-Hage, N., Rodriguez, M., Dever, S.M., Masvekar, R.R., Gewirtz, D.A. & Shacka, J.J. (2015) HIV-1 and Morphine Regulation of Autophagy in Microglia: Limited Interactions in the Context of HIV-1 Infection and Opioid Abuse J Virol. 89
3.) Yi L, Xiao H, Xu M, Ye X, Hu J, Li F, Li M, Luo C, Yu S, Bian X, Feng H. (2011) "Glioma-initiating cells: A predominant role in microglia/macrophages tropism to glioma." J Neuroimmunol. 232: 75-82.
4.) Kostianovsky AM, Maier LM, Anderson RC, Bruce JN, Anderson DE. (2008) "Astrocytic regulation of human monocytic/microglial activation." J Immunol. 181: 5425-32.
5.) Wang X, Douglas SD, Song L, Wang YJ, Ho WZ. (2008) "Neurokinin-1 receptor antagonist (aprepitant) suppresses HIV-1 infection of microglia/macrophages." J Neuroimmune Pharmacol. 3: 257-64.
6.) Campbell GR, Watkins JD, Singh KK, Loret EP, Spector SA. (2007) "Human Immunodeficiency Virus Type 1 Subtype C Tat Fails To Induce Intracellular Calcium Flux and Induces Reduced Tumor Necrosis Factor Production from Monocytes." J Virol. 81: 5919-28.
7.) Sui Z, Sniderhan LF, Schifitto G, Phipps RP, Gelbard HA, Dewhurst S, Maggirwar SB. (2007) "Functional Synergy between CD40 Ligand and HIV-1 Tat Contributes to Inflammation: Implications in HIV Type 1 Dementia." J Immunol. 178: 3226-36.
8.) Cassiani-Ingoni R, Cabral ES, L nemann JD, Garza Z, Magnus T, Gelderblom H, Munson PJ, Marques A, Martin R. (2006) "Borrelia burgdorferi induces TLR1 and TLR2 in human microglia and peripheral blood monocytes but differentially regulates HLA-Class II expression." J Neuropathol Exp Neurol. 65: 540-8.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1901 | MM | Microglia Medium |
$139.00
As low as:
$90.00
|
