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- Human Middle Ear Epithelial Cells
The middle ear is composed of three auditory ossicles (incus, malleus, and stapes), the tympanic membrane, and the Eustachian tube. The middle ear functions to transfer acoustic energy from the tympanic membrane to the cochlea. Middle Ear Epithelial Cells (MEEpiC) play an important role in homeostasis and sterility of the middle ear. Abnormalities associated with MEEpiC can lead to the development of middle ear disorders such as otitis media and cholesteatoma. Evidence indicates that epithelial cell proliferation is the underlying cause of cholesteatoma. Recent studies also suggest that the abnormal epithelial cell proliferation may be linked to inflammatory factors produced by middle ear fibroblasts. Human MEEpiC (HMEEpiC) are a useful in vitro model for studying middle ear diseases and may be used to identify new therapeutic targets for otitis media and cholesteatoma.
HMEEpiC from ScienCell Research Laboratories are isolated from human middle ear. HMEEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HMEEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HMEEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMEEpiC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium-2 (EpiCM-2 #4121) for culturing HMEEpiC in vitro.
| Catalog No. | 2460 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HMEEpiC |
| Size/Quantity | 5 x 10⁵ cells/vial |
| Product Use | HMEEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. Storage |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen, and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
2460No Publication available at this time
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4121 | EpiCM-2 | Epithelial Cell Medium-2 |
$122.00
As low as:
$81.00
|
||
| REAGENTS | |||||
| 0183 | T/E | Trypsin/EDTA Solution (0.05%; 100ml) |
$15.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
||
| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
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| 0923 | EpiF | EpiFectagen |
$253.00
|
