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- Human Myometrial Microvascular Endothelial Cells
Myometrial microvascular endothelial cells (MMEC) are located in the myometrium between the endometrium and the serosa of the uterus and exhibit typical cobblestone morphology. During pregnancy, angiogenesis occurs through MMEC proliferation in order to support the hypertrophy of the myometrial smooth muscle cell layer. Significant evidence also indicates that endothelial cell dysfunction in the myometrium may contribute to the development of preeclampsia. In addition, MMEC proliferation and angiogenesis may play a role in the formation of leiomyomata. Studies have shown that MMEC express the oxytocin receptor, which may promote vasodilation in the microvasculature of the myometrium. Human MMEC are a useful in vitro model to elucidate the mechanisms of angiogenesis and to develop treatments for female reproductive tract disorders.
HMMEC from ScienCell Research Laboratories are isolated from human uterus. HMMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HMMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HMMEC in vitro.
| Catalog No. | 7000 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HMMEC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Gargett C, Bucak K, Rogers P. (2000) "Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells." Hum Reprod. 15(2): 293-301.
[2] Hayman R, Brockelsby J, Kenny L, Baker P. (1999) "Preeclampsia: the endothelium, circulating factor(s) and vascular endothelial growth factor." J So Gynecol Investig. 6(1): 3-10. [3] Weston G, Cann L, Rogers P. (2003) "Myometrial microvascular endothelial cells express oxytocin receptor." BJOG. 110(2):149-156. |
7000No Publication available at this time
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
|
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
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| MOLECULAR BIOLOGY | |||||
| 7004 | HMMEC cDNA | Human Myometrial Microvascular Endothelial Cell cDNA |
$453.00
|
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| 7009 | HMMEC gDNA | Human Myometrial Microvascular Endothelial Cell Genomic DNA |
$438.00
|
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