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Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The brain is known to contain about 1 x 10^11 neurons, each being able to contact at least 10,000 other neurons [2]. Brain stem neurons provide main motor and sensory innervation and integrative functions by regulating heart rate, breathing, sleep cycle, awareness, consciousness, and more [3]. Neurons isolated from different brain regions serve as excellent models for studies of region-specific pathologies and degeneration, neurotoxicology and brain development [4].
HN-bs from ScienCell Research Laboratories are isolated from human brain stem. HN-bs are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HN-bs are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and β-tubulin III. HN-bs are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HN-bs are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HN-bs are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use neuronal medium (NM, Cat. #1521) for culturing HN-bs in vitro.
Catalog No. | 1560 |
---|---|
Country of Manufacture | United States |
Product Code | HN-bs |
Size/Quantity | 1 x 10^6 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins,
Quebec, Canada.[2] Alberts, B., Bray, D., Lewis, J., Raff, M., et. al. (1989) Molecular biology of the cell. 2nd ed., New York:
Garland. [3] Smith, J., Smith, A, Abdala, H., Koizumi, I., et al. (2008) J Neurophysiol. 98: 3370-87. [4] Zheng, Z., Lewis, M., Alberto Travagli, R. (2005) Am J Physiol Gastrointest Liver Physiol. 288: G1066-73. [5] Mitchell, J., Paul, P., Chen, H., Morris, A., et. al. (2010) Proc Natl Acad Sci U S A. 107:7556-61. [6] Wang, C., Sadovova, N., Ali, H.K., Duhart, H.M., et. al. (2007) Neuroscience. 144:46-55. [7] Sato, H., Fukutani, Y., Yamamoto, Y., Tatara, E., et. al. (2012) J Neurosci. 32:15388-402. |
No Publication available at this time
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
---|---|---|---|---|---|
MEDIA | |||||
1521 | NM | Neuronal Medium |
$126.00
As low as:
$81.00
|
||
REAGENTS | |||||
0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
||
0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
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