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The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphologic features, the key elements of a highly complex communication network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The human brain contains about 1 x 10^11 neurons, each being able to contact at least 10,000 other neurons [2].
HN from ScienCell Research Laboratories are isolated from human brain. HN are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HN are characterized by immunofluorescence with antibodies specific to β-tubulin III. HN are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HN are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HN are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Neuronal Medium (NM, Cat. #1521) for culturing HCGC in vitro.
| Catalog No. | 1520 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HN |
| Size/Quantity | 1 x 10^6 cells/vial / 5 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada. [2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland. |
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Easy to use cortical neurons Review by MC
Ease-of-use Overall quality of the product Technical resources I use a different neuronal media with these neurons so that I have more options of removed ingredients for the basal media. The neurons have some astrocytes and oligodendrocytes in the culture. They're all post-mitotic, but the health of the culture is very biased by confluency. The neurons are fairly sensitive to environmental changes, but only require simple care techniques. (Posted on 5/18/2017)
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Easy to use neurons for my research Review by Melinda R.
Ease-of-use Overall quality of the product Technical resources I work with neurons but needed a new source to purchase them after my last experience with a different company. These neurons worked well for me. They came cryopreserved at P0 and were competitively priced. Thanks, Sciencell. (Posted on 5/12/2017)
1.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12
2.) Olsson, M., Hultman, K., Dunoyer-Geindre, S., Curtis, M.A., Faull, R.L.M., Kruithof, E.K.O. & Jern, C. (2016) Epigenetic Regulation of Tissue-Type Plasminogen Activator in Human Brain Tissue and Brain-Derived Cells Gene Regul Syst Bio. 10
3.) Watanabe, S., Ohno, S., Shirogane, Y., Suzuki, S.O., Koga, R. & Yanagi, Y. (2015) Measles Virus Mutants Possessing the Fusion Protein with Enhanced Fusion Activity Spread Effectively in Neuronal Cells, but Not in Other Cells, without Causing Strong Cytopathology J Virol. 89
4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
5.) Chu S, Ma Y, Feng D, Li Z, Jiang P. (2013) "Predictive value of the SLC22A18 protein expression in glioblastoma patients receiving temozolomide therapy." J Transl Med. 11: 69.
6.) Agudelo, M., Yoo, C. & Nair, M.P. (2012) Alcohol-induced serotonergic modulation: the role of histone deacetylases Alcohol. 46
7.) Deshmane SL, Mukerjee R, Fan S, Sawaya BE. (2011) "High-performance capillary electrophoresis for determining HIV-1 Tat protein in neurons." PLoS One. 6: e16148.
8.) Saiyed ZM, Gandhi N, Agudelo M, Napuri J, Samikkannu T, Reddy PV, Khatavkar P, Yndart A, Saxena SK, Nair MP. (2011) "HIV-1 Tat upregulates expression of histone deacetylase-2 (HDAC2) in human neurons: Implication for HIV-associated neurocognitive disorder (HAND)." Neurochem Int. 58: 656-64.
9.) Gouras GK, Tampellini D, Takahashi RH, Capetillo-Zarate E. (2010) "Intraneuronal beta-amyloid accumulation and synapse pathology in Alzheimer's disease." Acta Neuropathol. 119: 523-41.
10.) Mak YT, Lam WP, L L, Wong YW, Yew DT. (2010) "The toxic effect of ketamine on SH-SY5Y neuroblastoma cell line and human neuron." Microsc Res Tech. 73: 195-201.
11.) Lee CT, Lehrmann E, Hayashi T, Amable R, Tsai SY, Chen J, Sanchez JF, Shen J, Becker KG, Freed WJ. (2009) "Gene Expression Profiling Reveals Distinct Cocaine-Responsive Genes in Human Fetal CNS Cell Types." J Addict Med. 3: 218-26.
12.) Lee CT, Chen J, Hayashi T, Tsai SY, Sanchez JF, Errico SL, Amable R, Su TP, Lowe RH, Huestis MA, Shen J, Becker KG, Geller HM, Freed WJ. (2008) "A mechanism for the inhibition of neural progenitor cell proliferation by cocaine." PLoS Med. 5: e117
13.) Karmakar S, Banik NL, Patel SJ, Ray SK. (2007) "Garlic compounds induced calpain and intrinsic caspase cascade for apoptosis in human malignant neuroblastoma SH-SY5Y cells." Apoptosis. 12: 671-84.
14.) Michaelis M, Kleinschmidt MC, Doerr HW, Cinatl J Jr. (2007) "Minocycline inhibits West Nile virus replication and apoptosis in human neuronal cells." J Antimicrob Chemother. 60: 981-6.
15.) Cassiani-Ingoni R, Cabral ES, L?nemann JD, Garza Z, Magnus T, Gelderblom H, Munson PJ, Marques A, Martin R. (2006) "Borrelia burgdorferi induces TLR1 and TLR2 in human microglia and peripheral blood monocytes but differentially regulates HLA-Class II expresssion." Journal of Neupathology & Experimental Neurology. 65: 540-48.
16.) Padmanabhan J, Levy M, Dickson DW, Potter H. (2006) "Alpha1-antichymotrypsin, an inflammatory protein overexpressed in Alzheimer's disease brain, induces tau phosphorylation in neurons." Brain. 129: 3020-34.
17.) Hashimoto M, Bar-On P, Ho G, Takenouchi T, Rockenstein E, Crews L, Masliah E. (2004) "Synuclein Regulates Akt Activity in Neuronal Cells-a possible mechanism for neuroprotecton in parkinson's disease." J Biol Chem. 279: 23622-9.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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Product Sheets
1520-5 (5 Million Cells per Vial)
1520-10 (10 Million Cells per Vial)
Certificates of Analysis
For a specific lot number, please search below:
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1521 | NM | Neuronal Medium |
$126.00
As low as:
$81.00
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| MOLECULAR BIOLOGY | |||||
| 1529 | HN gDNA | Human Neuron genomic DNA |
$398.00
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| 1526 | HN Lysate | Human Neuron Lysate |
$398.00
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| 1524 | HN cDNA | Human Neuron cDNA |
$411.00
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| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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