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- Human Non-Pigmented Ciliary Epithelial Cells
The ciliary epithelium rests upon the leaf-like projections of the ciliary processes in the posterior chamber, overlaying the core of stroma and blood vessels. It consists of two layers of columnar cells, which appear structurally and functionally distinct. The outer layer of epithelium is pigmented, but the inner layer, which resides next to the aqueous, is not. In mammalian eyes, the non-pigmented ciliary epithelial cells (NPCEpiC) are largely responsible for the aqueous humor inflow. NPCEpiC generate an osmotic gradient through solute transfer, which then results in water movement from the stroma into the posterior chamber. Other studies have implicated NPCEpiC in the synthesis of macro-molecules required for photo-transduction and the development of rare adenomas in the ciliary body.
HNPCEpiC from ScienCell Research Laboratories are isolated from human ciliary body. HNPCEpiC are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HNPCEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HNPCEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HNPCEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for culturing HNPCEpiC in vitro.
Catalog No. | 6580 |
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Country of Manufacture | United States |
Product Code | HNPCEpiC |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1]. Krupin, T. and Civan, M. M. (1995) in The Glaucomas, eds. Ritch, R., Shields, M. B. & Krupin, T. (Mosby, St. Louis), pp. 251-280. [2]. Raviola, G. and Raviola, E. (1978) Intercellular junctions in the ciliary epithelium. Investigative Ophthalmology & Visual Science 17:958-981. |
1.) Yamanouchi K, Tsuruga E, Oka K, Sawa Y, Ishikawa H. (2012) "Fibrillin-1 and fibrillin-2 are essential for formation of thick oxytalan fibers in human nonpigmented ciliary epithelial cells in vitro." Connect tissue res.53: 14-20.
2.) Kalariya NM, Shoeb M, Reddy AB, Zhang M, van Kuijk FJ, Ramana KV. (2010) "Prevention of endotoxin-induced uveitis in rats by plant sterol guggulsterone." Invest Ophthalmol Vis Sci. 51: 5105-13.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
4101 | EpiCM | Epithelial Cell Medium |
$135.00
As low as:
$90.00
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MOLECULAR BIOLOGY | |||||
6584 | HNPCEpiC cDNA | Human Non-Pigment Ciliary Epithelial Cell cDNA |
$453.00
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6585 | HNPCEpiC tRNA | Human Non-Pigmented Ciliary Epithelial Cell Total RNA |
$401.00
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6586 | HNPCEpiC Lysate | Human Non-Pigment Ciliary Epithelial Cell Lysate |
$438.00
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6587 | HNPCEpiC miRNA | Human Non-Pigment Ciliary Epithelial Cell MicroRNA |
$374.00
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6589 | HNPCEpiC gDNA | Human Non-Pigmented Ciliary Epithelial Cell Genomic DNA |
$438.00
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