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- Human Oral Keratinocytes
Oral keratinocytes act as the major barrier to physical, microbial, and chemical agents that may cause local cell injury. They are involved in the proinflammatory process through the production of cytokines either constitutively or after a variety of stimuli. Consequently, oral keratinocytes may potentially participate in controlling oral infections through an inflammatory process involving different interleukins, such as IL-1β and IL-18. Oral keratinocytes express a variety of differentiation markers, the expression of which is influenced by calcium-induced changes in the transcription of target genes. Oral keratinocytes share major structural and functional features with the well-characterized dermal keratinocyte and are a good model to study basic keratinocyte biology, as well as, processes of immortalization and malignant transformation.
HOK from ScienCell Research Laboratories are isolated from human oral mucosa. HOK are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HOK are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HOK are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOK are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Oral Keratinocyte Medium (OKM, Cat. No. 2611) for the culturing of HOK in vitro.
Catalog No. | 2610 |
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Country of Manufacture | United States |
Product Code | HOK |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1]. Lundqvist, C., Baranov, V., Teglund, S., Hammarstrom, S. and Hammarstrom, M.L. (1994) Cytokine profile and ultrastructure of intraepithelial gamma delta T cells in chronically inflamed human gingiva suggest a cytotoxic effector function. J. Immunol. 153:2302-2312. [2]. M. Rouabhia, G. Ross, N. Page, and J. Chakir (2002) Interleukin-18 and Gamma Interferon Production by Oral Epithelial Cells in Response to Exposure to Candida albicans or Lipopolysaccharide Stimulation. Infect. Immun. 70:7073-7080. [3]. Presland, R.B. and Dale, B.A. (2000) Epithelial structural proteins of the skin and oral cavity: function in health and disease. Crit. Rev. Oral Biol. Med. 11:383-408. |
Product Sheets
Safety Data Sheets
Please login or register to access the Safety Data Sheet for Human Oral KeratinocytesCertificates of Analysis
For a specific lot number, please search below:
1.) Utheim, T.P., Islam, R., Fostad, I.G., Eidet, J.R., Sehic, A., Olstad, O.K., Dartt, D.A., Messelt, E.B., Griffith, M. & Pasovic, L. (2016) Storage Temperature Alters the Expression of Differentiation-Related Genes in Cultured Oral Keratinocytes PLoS One. 11
2.) Ho, M.H., Huang, L., Goodwin, J.S., Dong, X., Chen, C.H. & Xie, H. (2016) Two Small Molecules Block Oral Epithelial Cell Invasion by Porphyromons gingivalis PLoS One. 11
3.) Wu, B., Lei, D., Wang, L., Yang, X., Jia, S., Yang, Z., Shan, C., Yang, X., Zhang, C. & Lu, B.(2016) 'MiRNA101 inhibits oral squamouscell carcinoma growth and metastasis by targeting zinc finger Ebox binding homeobox 1' American Journal of Cancer Research. VOL 6
4.) Peng, H.Y., Cheng, Y.C., Hsu, Y.M., Wu, G.H., Kuo, C.C., Liou, J.P., Chang, J.Y., Jin, S.L.C. & Shiah, S.G.(2016) 'MPT0B098, a Microtubule Inhibitor, Suppresses JAK2/STAT3 Signaling Pathway through Modulation of SOCS3 Stability in Oral Squamous Cell Carcinoma' PLoS One. VOL 11
5.) Yang, J., Wei, D., Wang, W., Shen, B., Xu, S. & Cao, Y. (2015) TRAF4 enhances oral squamous cell carcinoma cell growth, invasion and migration by Wnt-?-catenin signaling pathway Int J Clin Exp Pathol. 8
6.) Miller, J.H., Avil‚s-Reyes, A., Scott-Anne, K., Gregoire, S., Watson, G.E., Sampson, E., Progulske-Fox, A., Koo, H., Bowen, W.H., Lemos, J.A. & Abranches, J. (2015) The Collagen Binding Protein Cnm Contributes to Oral Colonization and Cariogenicity of Streptococcus mutans OMZ175 Infect Immun. 83
7.) Lin, H.Y., Hung, S.K., Lee, M.S., Chiou, W.Y., Huang, T.T., Tseng, C.E., Shih, L.Y., Lin, R.I., Lin, J.M., Lai, Y.H., Chang, C.B., Hsu, F.C., Chen, L.C., Tsai, S.J., Su, Y.C., Li, S.C., Lai, H.C., Hsu, W.L., Liu, D.W., Tai, C.K., Wu, S.F. & Chan, M.W. (2015) DNA methylome analysis identifies epigenetic silencing of FHIT as a determining factor for radiosensitivity in oral cancer: an outcome-predicting and treatment-implicating study Oncotarget. 6
8.) Li, T., Li, L., Li, D., Wang, S. & Sun, J. (2015) MiR-34a inhibits oral cancer progression partially by repression of interleukin-6-receptor Int J Clin Exp Pathol. 8
9.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
10.) Lee, C.H., Pan, K.L., Tang, Y.C., Tsai, M.H., Cheng, A.J., Shen, M.Y., Cheng, Y.M., Huang, T.T. & Lin, P. (2015) LDOC1 silenced by cigarette exposure and involved in oral neoplastic transformation Oncotarget. 6
11.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) 'Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells.' Mol cancer.
12.) Yen YC, Shiah SG, Chu HC, Hsu YM, Hsiao JR, Chang JY, Hugn WC, Liao CT, Cheng AJ, Lu YC, Chen YW. (2014) "Reciprocal regulation of MicroRNA-99a and insulin-like growth factor I receptor signaling in oral squamous cell carcinoma cells." Mol cancer. 13: 6.
13.) Ji W, Lee C, Chen JY, Cheng Y, Yang S, Chen J, Chen H. (2013) "Areca Nut Extract Induces Pyknotic Necrosis in Serum-Starved Oral Cells via Increasing Reactive Oxygen Species and Inhibiting GSK3: An Implication for Cytopathic Effects in Betel Quid Chewers." PLoS One. 8: e63295.
14.) Wallet MA, Calderon NL, Alonso TR, Choe CS, Catalfamo DL, Lalane CJ, Naiva KG, Panagakos F, Wallet SM. (2013) ?Triclosan alters antimicrobial and inflammatory responses of epithelial cells.? Oral dis. 19: 296-302.
15.) Talwar S, Balasubramanian S, Sundaramurthy S, House R, Wilusz CJ, Kuppuswamy D, D'Silva N, Gillespie MB, Hill EG, Palanisamy V. (2013) "Overexpression of RNA-binding protein CELF1 prevents apoptosis and destabilizes pro-apoptotic mRNAs in oral cancer cells." RNA Biol. 10: 277-86.
16.) Lee SS, Tsai CH, Yu CC, Chang YC. (2013) "Elevated Snail Expression Mediates Tumor Progression in Areca Quid Chewing-Associated Oral Squamous Cell Carcinoma via Reactive Oxygen Species." PLoS One. 8: e67985.
17.) Kim JS, Yu SK, Lee MH, Park MG, Park E, Kim SG, Lee SY, Kim CS, Kim HJ, Chun HS, Chun SW, Kim DK. (2013) 'MicroRNA-205 directly regulates the tumor suppressor, interleukin-24, in human KB oral cancer cells.'
18.) Kim JS, Yu SK, Lee MH, Park MG, Park E, Kim SG, Lee SY, Kim CS, Kim HJ, Chun HS, Chun SW, Kim DK. (2013) "MicroRNA-205 directly regulates the tumor suppressor, interleukin-24, in human KB oral cancer cells." Mol cells. 35: 17-24.
19.) Russo N, Wang X, Liu M, Banerjee R, Goto M, Scanlon C, Metwally t, Inglehart RC, Tsodikov A, Duffy S, Tubergen EV, Bradford C, Carey T, Wolf G, Chinnaiyan AM, D
20.) Kumar B, Yadav A, Lang JC, Cipolla MJ, Schmitt AC, Arradaza N, Teknos TN, Kumar P. (2012) 'YM155 reverses cisplatin resistance in head and neck cancer by decreasing cytoplasmic survivin levels.'
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
2611 | OKM | Oral Keratinocyte Medium |
$136.00
As low as:
$90.00
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MOLECULAR BIOLOGY | |||||
2614 | HOK cDNA | Human Oral Keratinocyte cDNA |
$453.00
|
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2615 | HOK tRNA | Human Oral Keratinocyte Total RNA |
$401.00
|
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2616 | HOK Lysate | Human Oral Keratinocyte Lysate |
$438.00
|
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2617 | HOK miRNA | Human Oral Keratinocyte MicroRNA |
$374.00
|
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2619 | HOK gDNA | Human Oral Keratinocyte genomic DNA |
$438.00
|