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- Human Pericardial Fibroblasts
Pericardial Fibroblasts (PcF) are the major cell type of the pericardium. They produce collagens and help form the thin but strong pericardium to serve as a protective sac for the heart by providing structural support and lubrication, and preventing excessive dilation of the heart [1, 2]. Dysregulated activation and proliferation of PcF, collagen and fibrin deposition, chronic inflammation, and scarring have been implicated in constrictive pericarditis, where pericardium hardening eventually prevents the heart from expanding [3, 4]. Associations between fibroblast activation and immunomodulation make PcF cultures a useful tool for studying progressive sclerosing pericarditis.
HPcF from ScienCell Research Laboratories are isolated from the human heart. HPcF are cryopreserved at passage one and are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPcF are characterized by immunofluorescence with antibody specific to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPcF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HPcF in vitro.
| Catalog No. | 6430 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HPcF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keepthe cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-
50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981. [2] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. |
6430No Publication available at this time
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
|
||
| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
||
| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
||
| 0913 | FibroF | FibroFectagen |
$253.00
|
||
| MOLECULAR BIOLOGY | |||||
| 6434 | HPcF cDNA | Human Pericardial Fibroblast cDNA |
$411.00
|
||
| 6435 | HPcF tRNA | Human Pericardial Fibroblast Total RNA |
$364.00
|
||
| 6439 | HPcF gDNA | Human Pericardial Fibroblast Genomic DNA |
$398.00
|
||
