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- Human Periodontal Ligament Fibroblasts
The periodontal ligament is connective tissue located between the cementum of the teeth and the alveolar bone of the mandibula. It plays an integral role in the maintenance and regeneration of periodontal tissue. The cells responsible for maintaining this tissue are thought to be fibroblasts, which can be either multipotent or composed of heterogeneous cell populations that differ in their function. Despite the similar morphology with gingival fibroblasts, periodontal ligament fibroblasts (PLF) appear to display distinct functional activities in the maintenance of tissue integrity. It is known that PLF produce osteoblast-related extracellular matrix proteins and show higher alkaline phosphatase activity compared with gingival fibroblasts. PLF actively participate in immune and inflammatory events in periodontal diseases.
HPLF from ScienCell Research Laboratories are isolated from human periodontal tissue. HPLF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPLF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HPLF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPLF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HPLF in vitro.
| Catalog No. | 2630 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HPLF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Han, X., Amar, S. (2002) Identification of genes differentially expressed in cultured human periodontal ligament fibroblasts vs. human gingival fibroblasts by DNA microarray analysis. J Dent Res. 81(6):399-405. [2] Murakami, Y., Kojima, T., Nagasawa, T., Kobayashi, H., Ishikawa, I. (2003) Novel isolation of alkaline phosphatase-positive subpopulation from periodontal ligament fibroblasts. J Periodontol. 74(6):780-6. [3] Takashiba, S., Naruishi, K., Murayama, Y. (2003) Perspective of cytokine regulation for periodontal treatment: fibroblast biology. J Periodontol. 74(1):103-10. |
26301.) Hong, L., Sharp, T., Khorsand, B., Fischer, C., Eliason, S., Salem, A., Akkouch, A., Brogden, K. & Amendt, B.A.(2016) 'MicroRNA200c Represses IL6, IL8, and CCL5 Expression and Enhances Osteogenic Differentiation' PLoS One. VOL 11
2.) Berahim, Z., Moharamzadeh, K., Jowett, A.K. & Rawlinson, A. (2015) Evaluation of Osteogenic and Cementogenic Potential of Periodontal Ligament Fibroblast Spheroids Using a Three-Dimensional In Vitro Model of Periodontium Int J Dent. 2015
3.) Premaraj S, Souza I, Premaraj T. (2011) "Mechanical loading activates beta-catenin signaling in periodontal ligament cells." Angle Orthod. 81: 592-9.
4.) Seo T, Cha S, Woo KM, Park YS, Cho YM, Lee JS, Kim TI. (2011) "Synergic induction of human periodontal ligament fibroblast cell death by nitric oxide and N-methyl-D-aspartic acid receptor antagonist." J Periodontal Implant Sci. 41: 17-22.
5.) Choi EJ, Yim JY, Koo KT, Seol YJ, Lee YM, Ku Y, Rhyu IC, Chung CP, Kim TI. (2010) "Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts." J Periodontal Implant Sci. 40: 105-10.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 2634 | HPLF cDNA | Human Periodontal Ligament Fibroblast cDNA |
$453.00
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| 2635 | HPLF tRNA | Human Periodontal Ligament Fibroblast Total RNA |
$401.00
|
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| 2636 | HPLF Lysate | Human Periodontal Ligament Fibroblast Lysate |
$438.00
|
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| 2637 | HPLF miRNA | Human Periodontal Ligament Fibroblast MicroRNA |
$374.00
|
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| 2639 | HPLF gDNA | Human Periodiontal Ligament Fibroblast genomic DNA |
$438.00
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