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- Human Preadipocytes-subcutaneous
Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissue and can proliferate and differentiate into mature adipocytes, contributing to increased adipose tissue mass. In vitro studies indicate that various tissue-derived preadipocytes exhibit different lipid accumulations, adipogenic transcription factor expression, and TNFα-induced apoptosis. There is also a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, obesity, diabetes, hyperlipidemia, and breast cancer.
HPA-s from ScienCell Research Laboratories are isolated from human subcutaneous fat tissue. HPA-s are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPA-s are characterized by immunofluorescence with antibodies specific to CD44 or CD90, and lipid staining after differentiation. HPA-s are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-s are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Preadipocyte Medium (PAM, Cat. #7211) for culturing HPA-s in vitro. Preadipocyte Differentiation Medium (PADM, Cat. #7221) can be used for in vitro differentiation of preadipocytes into mature adipocytes, and then followed by Adipocyte Medium (AdM, Cat. #7201), which maintains mature adipocytes after differentiation.
| Catalog No. | 7220 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HPA-s |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Tominaga, K., Johmura, Y., Nishizuka, M., Imagawa, M. (2004) Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation. J Cell Sci. 117(Pt 25):6217-26. [2] Reue, K., Glueck, S. B. (2001) Accumulating evidence for differences during preadipocyte development: Focus on "Differential gene expression in white and brown preadipocytes". Physiol Genomics. 7(1):1-2. [3] Tchkonia, T. et al. (2005) Abundance of Two Human Preadipocyte Subtypes with Distinct Capacities for Replication, Adipogenesis, and Apoptosis Varies among Fat Depots. Am J Physiol Endocrinol Metab. 288(1):E267-77. |
72201.) Li, X., Zhang, X., Shen, Z., Chen, Z., Wang, H., & Zhang, X. (2022). GnRH receptor mediates lipid storage in female adipocytes via AMPK pathway. International Journal of Medical Sciences, 19(9), 1442–1450. https://doi.org/10.7150/ijms.74335
2.) Yin, H., Qiu, X., Shan, Y., You, B., Xie, L., Zhang, P., Zhao, J., & You, Y. (2021). HIF‐1α downregulation of mir‐433‐3p in adipocyte‐derived exosomes contributes to NPC progression via targeting SCD1. Cancer Science, 112(4), 1457–1470. https://doi.org/10.1111/cas.14829
3.) Liu, G., Deng, Y., Song, Y., Sui, Y., Cen, J., Shao, Z., Li, H., & Tang, T. (2021). Transdermal delivery of adipocyte phospholipase A2 Sirna using microneedles to treat thyroid associated ophthalmopathy-related proptosis. Cell Transplantation, 30, 096368972110106. https://doi.org/10.1177/09636897211010633
4.) Sangaralingham, S. J., Whig, K., Peddibhotla, S., Kirby, R. J., Sessions, H. E., Maloney, P. R., Hershberger, P. M., Mose-Yates, H., Hood, B. L., Vasile, S., Pan, S., Zheng, Y., Malany, S., & Burnett, J. C. (2021). Discovery of Small Molecule Guanylyl cyclase a receptor positive allosteric modulators. Proceedings of the National Academy of Sciences, 118(52). https://doi.org/10.1073/pnas.2109386118
5.) Su, M. S., He, Y., Xue, S., Yu, J., Ren, X., Huang, N., Abdullahi, R., & Xu, M. (2021). Butyric acid alleviated chronic intermittent hypoxia-induced lipid formation and inflammation through up-regulating hur expression and inactivating AMPK Pathways. Bioscience Reports, 41(6). https://doi.org/10.1042/bsr20203639
6.) Nagao, H., Nishizawa, H., Tanaka, Y., Fukata, T., Mizushima, T., Furuno, M., Bamba, T., Tsushima, Y., Fujishima, Y., Kita, S., Funahashi, T., Maeda, N., Mori, M., Fukusaki, E., & Shimomura, I. (2018). Hypoxanthine secretion from human adipose tissue and its increase in hypoxia. Obesity, 26(7), 1168–1178. https://doi.org/10.1002/oby.22202
7.) Lee, M. Y., Wang, Y., Mak, J. C., & Ip, M. S. (2016). Intermittent hypoxia induces NF-ΚB-dependent endothelial activation via adipocyte-derived mediators. American Journal of Physiology-Cell Physiology, 310(6). https://doi.org/10.1152/ajpcell.00240.2015
8.) Hu H, He ML, Tao R, Sun HY, Hu R, Zang WJ, Yuan BX, Lau CP, Tse HF, Li GR. (2009) "Characterization of ion channels in human preadipocytes. J Cell Physiol. 218: 427-35.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 7211 | PAM | Preadipocyte Medium |
$125.00
As low as:
$90.00
|
||
| 7221 | PADM | Preadipocyte Differentiation Medium |
$251.00
As low as:
$116.00
|
||
| 7201 | AdM | Adipocyte Medium |
$139.00
As low as:
$90.00
|
||
| MOLECULAR BIOLOGY | |||||
| 7224 | HPA-s cDNA | Human Preadipocyte-subcutaneous cDNA |
$453.00
|
||
| 7225 | HPA-s tRNA | Human Preadipocyte-subcutaneous Total RNA |
$401.00
|
||
| 7226 | HPA-s Lysate | Human Preadipocyte-subcutaneous Lysate |
$438.00
|
||
| 7227 | HPA-s miRNA | Human Preadipocyte-subcutaneous MicroRNA |
$374.00
|
||
| 7229 | HPA-s gDNA | Human Preadipocyte-subcutaneous Genomic DNA |
$438.00
|
||
