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- Human Preadipocytes-visceral
Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissue and can proliferate and differentiate into mature adipocytes, contributing to increased adipose tissue mass. In vitro studies indicate that various tissue-derived preadipocytes exhibit different lipid accumulations, adipogenic transcription factor expression, and TNFα-induced apoptosis. There is also a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, obesity, diabetes, hyperlipidemia, and breast cancer.
HPA-v from ScienCell Research Laboratories are isolated from human visceral fat tissue. HPA-v are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPA-v are characterized by immunofluorescence with antibodies specific to CD44 or CD90, and lipid staining after differentiation. HPA-v are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-v are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Preadipocyte Medium (PAM, Cat. #7211) for the culturing of HPA-v in vitro. Preadipocyte Differentiation Medium (PADM, Cat. #7221) can be used for in vitro differentiation of preadipocytes into mature adipocytes, and then followed by Adipocyte Medium (AdM, Cat. #7201), which maintains mature adipocytes after differentiation.
| Catalog No. | 7210 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HPA-v |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Tominaga, K., Johmura, Y., Nishizuka, M., Imagawa, M. (2004) Fad24, a mammalian homolog of Noc3p, is a positive regulator in adipocyte differentiation. J Cell Sci. 117(Pt 25):6217-26. [2] Reue, K., Glueck, S. B. (2001) Accumulating evidence for differences during preadipocyte development: Focus on "Differential gene expression in white and brown preadipocytes". Physiol Genomics. 7(1):1-2. [3] Tchkonia, T. et al. (2005) Abundance of Two Human Preadipocyte Subtypes with Distinct Capacities for Replication, Adipogenesis, and Apoptosis Varies among Fat Depots. Am J Physiol Endocrinol Metab. 288(1):E267-77. |
72101.) Liu, A., Pan, W., Zhuang, S., Tang, Y., & Zhang, H. (2022). Cancer cell-derived exosomal mir-425-3p induces white adipocyte atrophy. Adipocyte, 11(1), 487–500. https://doi.org/10.1080/21623945.2022.2108558
2.) Yu, C., Wen, Q., Ren, Q., Du, Y., & Xie, X. (2021). Polychlorinated biphenyl Congener 180 (PCB 180) regulates mitotic clonal expansion and enhances adipogenesis through modulation of C/EBPΒ SUMOylation in preadipocytes. Food and Chemical Toxicology, 152, 112205. https://doi.org/10.1016/j.fct.2021.112205
3.) Sangaralingham, S. J., Whig, K., Peddibhotla, S., Kirby, R. J., Sessions, H. E., Maloney, P. R., Hershberger, P. M., Mose-Yates, H., Hood, B. L., Vasile, S., Pan, S., Zheng, Y., Malany, S., & Burnett, J. C. (2021). Discovery of Small Molecule Guanylyl cyclase a receptor positive allosteric modulators. Proceedings of the National Academy of Sciences, 118(52). https://doi.org/10.1073/pnas.2109386118
4.) Feng, J., Zhang, X., Shan, C., Xia, J., Zhang, Z., Shi, H., Leng, K., Wu, Y., Ji, C., & Zhong, T. (2021). Src family kinases involved in the differentiation of human preadipocytes. Molecular and Cellular Endocrinology, 533, 111323. https://doi.org/10.1016/j.mce.2021.111323
5.) Lin, Y., Zhang, Y., Xu, L., Long, W., Shan, C., Ding, H., You, L., Zhao, C., & Shi, Z. (2021). High expression of an unknown long noncoding RNA RP11-290L1.3 from GDM macrosomia and its effect on preadipocyte differentiation. Endocrine Connections, 10(2), 191–204. https://doi.org/10.1530/ec-20-0584
6.) Chen, X., Wu, T., Gong, Z., Guo, J., Liu, X., Zhang, Y., Li, Y., Ferraro, P., & Li, B. (2021). Lipid droplets as endogenous intracellular microlenses. Light: Science & Applications, 10(1). https://doi.org/10.1038/s41377-021-00687-3
7.) Sun, W., Sun, X., Chu, W., Yu, S., Dong, F., & Xu, G. (2019). CircRNA expression profiles in human visceral preadipocytes and adipocytes. Molecular Medicine Reports. https://doi.org/10.3892/mmr.2019.10886
8.) Pan, D.-S., Wang, W., Liu, N.-S., Yang, Q.-J., Zhang, K., Zhu, J.-Z., Shan, S., Li, Z.-B., Ning, Z.-Q., Huang, L., & Lu, X.-P. (2017). Chiglitazar preferentially regulates gene expression via configuration-restricted binding and phosphorylation inhibition of PPARγ. PPAR Research, 2017, 1–16. https://doi.org/10.1155/2017/4313561
9.) Shi, C., Huang, F., Gu, X., Zhang, M., Wen, J., Wang, X., You, L., Cui, X., Ji, C., & Guo, X. (2016). Adipogenic MIRNA and meta-signature mirnas involved in human adipocyte differentiation and obesity. Oncotarget, 7(26), 40830–40845. https://doi.org/10.18632/oncotarget.8518
10.) Gu, N., You, L., Shi, C., Yang, L., Pang, L., Cui, X., Ji, C., Zheng, W. & Guo, X.(2016) 'Expression of miR199a3p in human adipocytes is regulated by free fatty acids and adipokines' Mol Med Rep. VOL 14
11.) Yang, L., Shi, C.-mei, Chen, L., Pang, L.-xia, Xu, G.-feng, Gu, N., Zhu, L.-jun, Guo, X.-rong, Ni, Y.-hui, & Ji, C.-bo. (2014). The biological effects of HSA-mir-1908 in human adipocytes. Molecular Biology Reports, 42(5), 927–935. https://doi.org/10.1007/s11033-014-3830-1
12.) Ye, Z.-W., Wu, X.-M., & Jiang, J.-G. (2009). Knockdown of angiotensinogen by shrna-mediated RNA interference inhibits human visceral preadipocytes differentiation. International Journal of Obesity, 34(1), 157–164. https://doi.org/10.1038/ijo.2009.197
13.) Ye ZW, Wu XM, Jiang JG. (2009) "Expression changes of angiotensin II pathways and bioactive mediators during human preadipocytes-visceral differentiation." Metabolism. 58: 1288-96.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 7211 | PAM | Preadipocyte Medium |
$125.00
As low as:
$90.00
|
||
| 7221 | PADM | Preadipocyte Differentiation Medium |
$251.00
As low as:
$116.00
|
||
| 7201 | AdM | Adipocyte Medium |
$139.00
As low as:
$90.00
|
||
| MOLECULAR BIOLOGY | |||||
| 7214 | HPA-v cDNA | Human Preadipocyte-visceral cDNA |
$453.00
|
||
| 7215 | HPA-v tRNA | Human Preadipocyte-visceral Total RNA |
$401.00
|
||
| 7216 | HPA-v Lysate | Human Preadipocyte-visceral Lysate |
$438.00
|
||
| 7217 | HPA-v miRNA | Human Preadipocyte-visceral MicroRNA |
$374.00
|
||
| 7219 | HPA-v gDNA | Human Preadipocyte-visceral Genomic DNA |
$438.00
|
||
