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The prostate is a major accessory organ of the male mammalian reproductive system, with structural and functional activities under the control of androgens. The prostate is composed of three compartments, which can be identified by the cytokeratin expression profile [1]. The basal cells express predominantly high molecular weight cytokeratins (CK5 and CK14), the luminal secretory cells express predominantly low molecular weight cytokeratins (CK8 and CK18) and the intermediate cells express a combination of basal and luminal cytokeratins [2]. Additionally, there is increasing evidence that basal epithelial cells differentiate into luminal epithelial cells. Prostate cancer is one of the most common cancers affecting men and it has a high morbidity and mortality rate. Prostate epithelial cell (PrEpiC) culture provides unique opportunities to study many important features of the prostate, as well as, chemical and hormonal carcinogenesis.
HPrEpiC from ScienCell Research Laboratories are isolated from normal human prostate tissue. HPrEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPrEpiC are characterized by immunofluorescence with antibodies specific to cytokeratine (CK-14, -18 and -19). HPrEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPrEpiC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommeneded Medium
It is recommended to use Prostate Epithelial Cell Medium (PEpiCM, Cat. #4411) for culturing HPrEpiC in vitro.
Catalog No. | 4410 |
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Country of Manufacture | United States |
Product Code | HPrEpiC |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] van Leenders G, Dijkman H, Hulsbergen-van de Kaa C, Ruiter D, Schalken J: Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. Lab Invest 2000, 80:1251-1258. [2] Sherwood ER, Berg LA, Mitchell NJ, McNeal JE, Kozlowski JM, Lee C: Differential cytokeratin expression in normal, hyperplastic and malignant epithelial cells from human prostate. J Urol 1990, 143:167-171. |
1.) Oh JH, Gertych A, Tajbakhsh J. (2013) "Nuclear DNA Methylation and Chromatin Condensation Phenotypes Are Distinct Between Normally Proliferating/Aging, Rapidly Growing/Immortal, and Senescent Cells." Oncotarget. 4: 474-93.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
4411 | PEpiCM | Prostate Epithelial Cell Medium |
$122.00
As low as:
$81.00
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REAGENTS | |||||
0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
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0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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0973 | EpiF | EpiFectagen II |
$253.00
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MOLECULAR BIOLOGY | |||||
4414 | HPrEpiC cDNA | Human Prostate Epithelial Cells cDNA |
$411.00
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4415 | HPrEpiC tRNA | Human Prostate Epithelial Cell Total RNA |
$364.00
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4416 | HPrEpiC Lysate | Human Prostate Epithelial Cell Lysate |
$398.00
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4417 | HPrEpiC miRNA | Human Prostate Epithelial Cell MicroRNA |
$340.00
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4419 | HPrEpiC gDNA | Human Prostate Epithelial Cell genomic DNA |
$398.00
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