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- Human Pulmonary Artery Endothelial Cells
The pulmonary artery carries deoxygenated blood from the heart to the lungs. Similar to other vascular endothelial cells, pulmonary artery endothelial cells (PAEC) modulate coagulation and fibrinolysis by producing activators and inhibitors of both processes. In addition, PAEC produce mediators that influence the adhesion and aggregation of blood platelets. PAEC also control vascular tone by synthesizing and processing vasoactive molecules. PAEC further interact with mesenchymal cells and regulate inflammatory events. Such functions of PAEC are implicated in acute lung injury, pulmonary hypertension, edema, embolism, pulmonary artery aneurysm and chronic obstructive lung disease.
HPAEC from ScienCell Research Laboratories are isolated from human pulmonary artery. HPAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HPAEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HPAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPAEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HPAEC in vitro.
| Catalog No. | 3100 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HPAEC |
| Size/Quantity | 5 x 10⁵ cells/vial |
| Product Use | HPAEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Wilson, C. N., Batra, V. K. (2002) Lipopolysaccharide binds to and activates A(1) adenosine receptors on human pulmonary artery endothelial cells. J. Endotoxin Res. 8(4):263-71. |
31001.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
2.) Zhu, S., Denman, C.J., Cobanoglu, Z.S., Kiany, S., Lau, C.C., Gottschalk, S.M., Hughes, D.P., Kleinerman, E.S. & Lee, D.A. (2015) The narrow-spectrum HDAC inhibitor entinostat enhances NKG2D expression without NK cell toxicity, leading to enhanced recognition of cancer cells Pharm Res. 32
3.) Rydkina E, Turpin LC, Sahni SK. (2010) "Rickettsia rickettsii infection of human macrovascular and microvascular endothelial cells reveals activation of both common and cell type-specific host response mechanisms." Infect Immun. 78: 2599-606.
4.) Shahani T, Lavend'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. (2010) "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool." Blood. 115: 4902-9.
5.) Moldobaeva A, Welsh-Servinsky LE, Shimoda LA, Stephens RS, Verin AD, Tuder RM, Pearse DB. (2006) "Role of protein kinase G in barrier-protective effects of cGMP in human pulmonary artery endothelial cells." Am J Physiol Lung Cell Mol Physiol. 290: L919-30.
6.) Zhou X, Perez F, Han K, Jurivich DA. (2006) "Clonal senescence alters endothelial ICAM-1 function." Mech Ageing Dev. 127: 779-85.
7.) Ray RB, Basu A, Steele R, Beyene A, McHowat J, Meyer K, Ghosh AK, Ray R. (2004) "Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells." Virology. 321: 181-8.
8.) Pearse DB, Shimoda LA, Verin AD, Bogatcheva N, Moon C, Ronnett GV, Welsh LE, Becker PM. (2003) "Effect of cGMP on lung microvascular endothelial barrier dysfunction following hydrogen peroxide." Endothelium. 10: 309-17.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
|
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
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| MOLECULAR BIOLOGY | |||||
| 3104 | HPAEC cDNA | Human Pulmonary Artery Endothelial Cell cDNA |
$453.00
|
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| 3105 | HPAEC tRNA | Human Pulmonary Artery Endothelial Cell Total RNA |
$401.00
|
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| 3106 | HPAEC Lysate | Human Pulmonary Artery Endothelial Cell Lysate |
$438.00
|
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| 3107 | HPAEC miRNA | Human Pulmonary Artery Endothelial Cell MicroRNA |
$374.00
|
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| 3109 | HPAEC gDNA | Human Pulmonary Artery Endothelial Cell genomic DNA |
$438.00
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