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- Human Renal Glomerular Endothelial Cells
Renal glomerular endothelial cells (GEC) are a specialized microvascular cell type involved in the regulation of glomerular ultrafiltration. They form the inner part of the filtration barrier and are involved in pathophysiological processes in the glomerulum. GEC constitutively produce bioactive molecules, which can be amplified by inflammatory and thrombotic molecules. Endothelial cell injury due to a severe glomerular lesion can inhibit angiogenesis and result in sclerosis at the injured site. GEC injury affects mesangial and epithelial cells and leads to the progression of renal disease. The biological properties of GEC remain largely unknown because of difficulties associated with the culturing, cloning and propagation of these cells.
HRGEC from ScienCell Research Laboratories are isolated from human kidneys. HRGEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HRGEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HRGEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRGEC are guaranteed to expand for 5 population doublings under the conditions specified by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HRGEC in vitro.
| Catalog No. | 4000 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HRGEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HRGEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Nangaku, M., Shankland, S. J., Couser, W. G. and Johnson, R. J. (1998) A new model of renal microvascular injury. Curr Opin Nephrol Hypertens 7(4):457-62. [2] Kester, M., Nowinski, R. J., Holthofer, H., Marsden, P. A. and Dunn, M. J. (1994) Characterization of platelet-activating factor synthesis in glomerular endothelial cell lines. Kidney Int 46(5):1404-12. [3] Lee, L. K., Meyer, T. W., Pollock, A. S. and Lovett, D. H. (1995) Endothelial cell injury initiates glomerular sclerosis in the rat remnant kidney. J Clin Invest 96(2):953-64. [4] Yamanaka, N. and Shimizu, A. (1999) Role of glomerular endothelial damage in progressive renal disease. Kidney Blood Press Res 22(1-2):13-20. |
40001.) Li, F., Zhang, J., Luo, L., & Hu, J. (2023). Protective effects of xanthohumol against diabetic nephropathy in a mouse model. Kidney and Blood Pressure Research. https://doi.org/10.1159/000528650
2.) Zhang, H., Li, L., Wang, Y., Dong, F., Chen, X., Liu, F., Xu, D., Yi, F., Kapron, C.M. & Liu, J.(2016) 'NFkB signaling maintains the survival of cadmiumexposed human renal glomerular endothelial cells' Int J Mol Med. VOL 38
3.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells." Int j mol sci. 15: 484-503.
4.) Park SW, Kim M, Chen SW, Brown KM, D'Agati VD, Lee HT. (2010) "Sphinganine-1-phosphate protects kidney and liver after hepatic ischemia and reperfusion in mice through S1P1 receptor activation." Lab Invest. 90: 1209-24.
5.) Zupancic ML, Frieman M, Smith D, Alvarez RA, Cummings RD, Cormack BP. (2008) "Glycan microarray analysis of Candida glabrata adhesin ligand specificity." Mol Microbiol. 68: 547-59.
6.) Advani A, Kelly DJ, Advani SL, Cox AJ, Thai K, Zhang Y, White KE, Gow RM, Marshall SM, Steer BM, Marsden PA, Rakoczy PE, Gilbert RE. (2007) "Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions." Proc Natl Acad Sci USA. 104: 14448-53.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
|
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
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| MOLECULAR BIOLOGY | |||||
| 4004 | HRGEC cDNA | Human Renal Glomerular Endothelial Cell cDNA |
$453.00
|
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| 4005 | HRGEC tRNA | Human Renal Glomerular Endothelial Cell Total RNA |
$401.00
|
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| 4006 | HRGEC Lysate | Human Renal Glomerular Endothelial Cell Lysate |
$438.00
|
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| 4007 | HRGEC miRNA | Human Renal Glomerular Endothelial Cell MicroRNA |
$374.00
|
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| 4009 | HRGEC gDNA | Human Renal Glomerular Endothelial Cell genomic DNA |
$438.00
|
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