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- Human Renal Proximal Tubular Epithelial Cells
Renal proximal tubular epithelial cells (PTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. PTEpiC are located near injury sites in a variety of congenital, metabolic, and inflammatory diseases. PTEpiC can produce inflammatory mediators such as cytokines and chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. PTEpiC express IL-2R alpha and MHC class II antigens during inflammation, after renal transplantation, or in crescentic glomerulonephritis. PTEpiC culture provides a useful in vitro model for studying the relationship between tubular cells and renal disease.
HRPTEpiC from ScienCell Research Laboratories are isolated from human kidney. HRPTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRPTEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18, -19 and vimentin. HRPTEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRPTEpiC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for the culturing of HRPTEpiC in vitro.
| Catalog No. | 4100 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HRPTEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] van Kooten, C., van der Linde, X., Woltman, A. M., van Es, L. A. and Daha, M. R. (1999) Synergistic effect of interleukin-1 and CD40L on the activation of human renal tubular epithelial cells. Kidney Int 56(1):41-51. [2] Schmouder, R. L., Strieter, R. M., Wiggins, R. C., Chensue, S. W. and Kunkel, S. L. (1992 ) In vitro and in vivo interleukin-8 production in human renal cortical epithelia. Kidney Int 41(1):191-8. [3] Wuthrich, R. P., Glimcher, L. H., Yui, M. A., Jevnikar, A. M., Dumas, S. E. and Kelley, V. E. (1990) MHC class II, antigen presentation and tumor necrosis factor in renal tubular epithelial cells. Kidney Int 37(2):783-92. |
1.) Chung, B.H., Kim, K.W., Kim, B.M., Doh, K.C., Cho, M.L. & Yang, C.W. (2015) Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction PLoS One. 10
2.) Van der Hauwaert C, Savary G, Gnemmi V, Glowacki F, Pottier N, Bouillez A, Maboudou P, Zini L, Leroy X, Cauffiez C, Perrais M, Aubert S. (2013) "Isolation?and?characterization?of a?primary?proximal?tubular?epithelial cell?model?from?human kidney?by?CD10/CD13?double?labeling." PLoS One.?8: e66750.
3.) Du T, Zou X, Cheng J, Wu S, Zhong L, Ju G, Zhu J, Liu G, Zhu Y, Xia S. (2013) "Human Wharton?s jelly-derived mesenchymal stromal cells reduce renal fibrosis through induction of native and foreign hepatocyte growth factor synthesis in injured tubular epithelial cells." Stem Cell Res Ther. 4: 59.
4.) Dudas PL, Sague SL, Elloso MM, Farrell FX. (2011) "Proinflammatory/Profibrotic Effects of Interleukin-17A on Human Proximal Tubule Epithelium." Nephron Exp Nephrol. 117: e114-23.
5.) Zhang H, Tasnim F, Ying JY, Zink D. (2009) "The impact of extracellular matrix coatings on the performance of human renal cells applied in bioartificial kidneys." Biomaterials. 30: 2899-911.
6.) Hatsukari I, Singh P, Hitosugi N, Messmer D, Valderrama E, Teichberg S, Chaung W, Gross E, Schmidtmayerova H, Singhal PC. (2007) "DEC-205-mediated internalization of HIV-1 results in the establishment of silent infection in renal tubular cells." J Am Soc Nephrol. 18: 780-7.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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4100| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4101 | EpiCM | Epithelial Cell Medium |
$122.00
As low as:
$81.00
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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| 0923 | EpiF | EpiFectagen |
$253.00
|
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| MOLECULAR BIOLOGY | |||||
| 4104 | HRPTEpiC cDNA | Human Renal Proximal Tubular Epithelial Cell cDNA |
$411.00
|
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| 4105 | HRPTEpiC tRNA | Human Renal Proximal Tubular Epithelial Cell Total RNA |
$364.00
|
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| 4106 | HRPTEpiC Lysate | Human Renal Proximal Tubular Epithelial Cell Lysate |
$398.00
|
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| 4107 | HRPTEpiC miRNA | Human Renal Proximal Tubular Epithelial Cell MicroRNA |
$340.00
|
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| 4109 | HRPTEpiC gDNA | Human Renal Proximal Tubular Epithelial Cell genomic DNA |
$398.00
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