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- Human Retinal Pigment Epithelial Cells
The retina is a multi-layered structure lining the posterior segment of the eye. It consists of photoreceptors and retinal pigment epithelial cells (RPEpiC). RPEpiC are situated between the neurosensory retina and the choroid, forming the outer blood-retina barrier that controls the chemical composition of the subretinal space [1]. RPEpiC have specific polarized protein distributions within the cell. In contrast to other epithelia which normally faces an apical lumen devoid of matrix, the RPEpiC apical plasma membrane is in direct contact with the extracellular matrix [2]. RPEpiC play a critical role in regulating the adjacent photoreceptor membrane turnover, retinoid metabolism, and protecting the retina from light-induced damages by the intracellular melanin [3], which suggests that RPEpiC may contribute to the pathogenesis of various ocular diseases such as proliferative retinopathy and age-related macular degeneration.
HRPEpiC from ScienCell Research Laboratories are isolated from human retina. HRPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRPEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18, -19 and fibronectin. HRPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HRPEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for culturing HRPEpiC in vitro.
| Catalog No. | 6540 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HRPEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Rizzolo, L. J. (1997) Polarity and the development of the outer blood-retinal barrier. Histol Histopathol 12:1057-1067. [2] Marmorstein, A. D. (2001) The polarity of the retinal pigment epithelium. Traffic 2:867-872. [3] Araki, M., Takano, T., Uemonsa, T., Nakane, Y., Tsudzuki, M., Kaneko, T. (2002) Epithelia-mesenchyme interaction plays an essential role in transdifferentiation of retinal pigment epithelium of silver mutant quail: localization of FGF and related molecules and aberrant migration pattern of neural crest cells during eye rudiment formation. Dev Biol. 244:358-71. |
1.) Favret S, Binet F, Lapalme E, Leboeuf D, Carbadillo J, Rubic T, Picard E, Mawambo G, Tetreault N, Jean- Joyal S, Chemtob S, Sennlaub F, SanGiovanni JP, Guimond M,Sapieha P. (2013) "Deficiency in the metabolite receptor SUCNR1 (GPR91) leads to outer retinal lesions." Aging (Albany NY). 5: 427.
2.) Miyamoto N, Izumi H, Miyamoto R, Bin H, Kondo H, Tawara A, Sasaguri Y, Kohno K. (2011) "Transcriptional Regulation of Activating Transcription Factor 4 under Oxidative Stress in Retinal Pigment Epithelial ARPE-19/HPV-16 Cells." Invest Ophthalmol Vis Sci. 52: 1226-34.
3.) Narayan S, Prasanna G, Tchedre K, Krishnamoorthy R, Yorio T. (2010) "Thrombin-induced endothelin-1 synthesis and secretion in retinal pigment epithelial cells is rho kinase dependent." J Ocul Pharmacol Ther. 26: 389-97.
4.) Bertram KM, Baglole CJ, Phipps RP, Libby RT. (2009) "Molecular regulation of cigarette smoke induced-oxidative stress in human retinal pigment epithelial cells: implications for age-related macular degeneration." Am J Physiol Cell Physiol. 297: C1200-10.
5.) Mannermaa E, Vellonen KS, Ryh?nen T, Kokkonen K, Ranta VP, Kaarniranta K, Urtti A. (2009) "Efflux protein expression in human retinal pigment epithelium cell lines." Pharm Res. 26: 1785-91.
6.) Cheong HI, Johnson J, Cormier M, Hosseini K. (2008) "In vitro cytotoxicity of eight beta-blockers in human corneal epithelial and retinal pigment epithelial cell lines: comparison with epidermal keratinocytes and dermal fibroblasts." Toxicol In Vitro. 22: 1070-6.
7.) Swaney JS, Moreno KM, Gentile AM, Sabbadini RA, Stoller GL. (2008) "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res. 87: 367-75.
8.) Hunt DW, Boivin WA, Fairley LA, Jovanovic MM, King DE, Salmon RA, Utting OB. (2006) "Ultraviolet B light stimulates interleukin-20 expression by human epithelial keratinocytes." Photochem Photobiol. 82: 1292-300.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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6540| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4101 | EpiCM | Epithelial Cell Medium |
$122.00
As low as:
$81.00
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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| 0923 | EpiF | EpiFectagen |
$253.00
|
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| MOLECULAR BIOLOGY | |||||
| 6544 | HRPEpiC cDNA | Human Retinal Pigment Epithelial Cell cDNA |
$411.00
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| 6545 | HRPEpiC tRNA | Human Retinal Pigment Epithelial Cell Total RNA |
$364.00
|
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| 6546 | HRPEpiC Lysate | Human Retinal Pigment Epithelial Cell Lysate |
$398.00
|
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| 6547 | HRPEpiC miRNA | Human Retinal Pigment Epithelial Cell MicroRNA |
$340.00
|
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| 6549 | HRPEpiC gDNA | Human Retinal Pigment Epithelial Cell Genomic DNA |
$398.00
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