GeneQuery™ Human SARS-CoV-2 Interacting Genes qPCR Array Kit

Coronaviruses are a family of large RNA viruses with sizes ranging from 26 to 32 kb. These viruses are zoonotic and in humans can cause respiratory infections. In December 2019, a new deadly coronavirus known as SARS-CoV-2, which has a high sequence similarity to SARS-CoV, was identified. SARS-CoV-2 has caused a pneumonia outbreak known as Covid-19 in Wuhan, China and spread globally. Currently, the limited understanding of the genetic basis of the virus-host cells interaction and the host’s response has become a major challenge in SARS-CoV-2 research and therapeutic treatments.

ScienCell's GeneQuery™ Human SARS-CoV-2 Interacting Genes qPCR Array Kit (GQH-SCV) is designed to facilitate gene expression profiling of 86 genes that were identified as SARS-CoV-2 interacting genes. Additionally, two SARS-CoV-2 genes, the nucleocapsid (N) and the spike (S) genes, were included in the panel allowing quantification of the virus presence in the samples. Brief examples of how included genes may be grouped according to their functions are shown below:      

• SAR-CoV2 virus genes: SARS-CoV-2 N gene, SARS-CoV-2 S gene
• Viral entry-associated proteins: ACE2, AGTR2, TMPRSS2, CTSL, CTSB
• Endocytosis: ACSL5, CRIP2, HSD17B4, EPHX1, MCCC2, GSTA4, ACACA, HGD
• Pulmonary surfactants: SFTPA1, SFTPB, SFTPC
• Pro-coagulation and profibrotic genes: PLSCR1, SERPINE1, THBS1, FGB, PLAT, PLAU, TGFB1, CTGF, PDGFA, COL10A1, COL11A1
• Innate immune signaling pathway: DDX58, IRF7, STAT1, G1P2, GBP1, IFNG, MX1, ISG20, OAS1, IDO1, IRAK3, NOS2, TNFSF10, RNF128, MCL1
• Inflammatory cytokines/chemokines and regulation: IL1A, IL1B, CXCL10, IL6, CXCL8, IL10, IL12A, CCL2, IFNG, IFIT1, TNF, G1P2, GBP1
• Cell cycle and development: AREG, CDK1, CDKN3, CKS2, CCNE1
• Epigenetic and gene expression regulators: CEP250, CEP350, CNTRL, NINL, PCNT, CDK5RAP2, CENPF, BRD2, HDAC2
• Protein translational machinery: EEF1A1, EIF4B, MIF4GD, DDX5
• Vesicle trafficking and protein degradation: GOLGA2, GOLGB1, GORASP1, GCC2, VPS39, SLC27A2, SLC25A21, ACADM, DCTN2
• Mitochondria functions: NDUFB9, NDUFAF2, NDUFA10, ABCC1, ALG11, MRPS27, MRPS5

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.