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- Human Schwann Cells
Schwann cells are neural crest derivatives that ensheathe and myelinate axons of peripheral nerves. Each Schwann cell wraps around the shaft of an individual peripheral axon, forming myelin sheaths along segments of the axon. Schwann cells play important roles in the development, function, and regeneration of peripheral nerves. When an axon is dying, the Schwann cells surrounding it aid in its digestion, leaving an empty channel formed by successive Schwann cells, through which a new axon may then grow from a severed end. The number of Schwann cells in peripheral nerves is tightly regulated. Their proliferation in vitro can be stimulated by various growth factors including PDGF, FGF, neuregulin, and others. Schwann cells provide a relatively simple, well-defined, and accessible mammalian model for the study of a number of developmental questions. It is also of particular clinical importance to understand the biology of Schwann cells, not only in the context of neuropathies and nerve regeneration, but also because the cells or their precursors may be especially well suited for implants to facilitate repair in the CNS.
HSC from ScienCell Research Laboratories are isolated from human spinal nerve. HSC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSC are characterized by immunofluorescence with antibodies specific to S100β. HSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HSC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories
Recommended Medium
It is recommended to use Schwann Cell Medium (SCM, Cat. #1701) for culturing HSC in vitro.
| Catalog No. | 1700 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HSC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Jessen, K. R. and Mirsky, R. (1999) Schwann cells and their precursors emerge as major regulators of nerve development. Trends Neurosci. 22:402-410. [2] Syroid, D. E., Maycox, P. R., Burrola, P. G., Liu, N., Wen, D., Lee, K. F., Lemke, G., Kilpatrick, T. J. (1996) Cell death in the Schwann cell lineage and its regulation by neuregulin. Proc. Natl. Acad. Sci. USA 93:9229-9234. [3] Rahmatullah, M., Schroering, A., Rothblum, K., Stahl, R. C., Urban, B and Carey, D. J. (1998) Synergistic regulation of Schwann cells proliferation by heregulin and forskolin. Mol. Cell. Biol. 18:6245-6252. |
17001.) Bedoui, Y., De Larichaudy, D., Daniel, M., Ah-Pine, F., Selambarom, J., Guiraud, P., & Gasque, P. (2022). Deciphering the role of Schwann cells in inflammatory peripheral neuropathies post alphavirus infection. Cells, 12(1), 100. https://doi.org/10.3390/cells12010100
2.) Merolli A, Mao Y, Voronin G, Steele JAM, Murthy NS, Kohn J. (2019). A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon. Neural Regen Res. 14(6):1052-1059.
3.) Su, F., Zhou, Z., Su, W., Wang, Z. & Wu, Q. (2016) 'A novel alternative splicing isoform of NF2 identified in human Schwann cells' Oncol Lett.
vol 12
4.) Lin, G., Zhang, H., Sun, F., Lu, Z., Reed-Maldonado, A., Lee, Y.C., Wang, G., Banie, L. & Lue, T.F. (2016) Brain-derived neurotrophic factor promotes nerve regeneration by activating the JAK/STAT pathway in Schwann cells Translational Andrology and Urology. 5
5.) Joshi, A.R., Holtmann, L., Bobylev, I., Schneider, C., Ritter, C., Weis, J. & Lehmann, H.C. (2016) 'Loss of Schwann cell plasticity in chronic inflammatory demyelinating polyneuropathy (CIDP)' J Neuroinflammation.
vol 13
6.) Su, F., Zhou, Z., Su, W., Wang, Z. & Wu, Q.(2016) 'A novel alternative splicing isoform of NF2 identified in human Schwann cells' Oncol Lett. VOL 12
7.) Joshi, A.R., Holtmann, L., Bobylev, I., Schneider, C., Ritter, C., Weis, J. & Lehmann, H.C.(2016) 'Loss of Schwann cell plasticity in chronic inflammatory demyelinating polyneuropathy (CIDP)' J Neuroinflammation. VOL 13
8.) Cho YR, Lim JH, Kim MY, Kim TW, Hong BY, Kim YS, Chang YS, Kim HW, Park CW. (2014) 'Therapeutic Effects of Fenofibrate on Diabetic Peripheral Neuropathy by Improving Endothelial and Neural Survival in db/db Mice.'
9.) Cho YR, Lim JH, Kim MY, Kim TW, Hong BY, Kim YS, Chang YS, Kim HW, Park CW. (2014) "Therapeutic Effects of Fenofibrate on Diabetic Peripheral Neuropathy by Improving Endothelial and Neural Survival in db/db Mice." PloS one. 9: e83204.
10.) Guerreiro LTA, Robottom-Ferreira AB, Ribeiro-Alves M, Toledo-Pinto TG, Brito TR, Rosa PS, Sandoval FG, Jardim MR, Antunes SG, Shannon EJ, Sarno EN, Pessolani MCV, Williams DL, Moraes MO. (2013) "Gene expression profiling specifies chemokine, mitochondrial and lipid metabolism signatures in leprosy." PLoS One. 8: e64748.
11.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2013) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15.
12.) Ramesh G, Santana-Gould L, Inglis FM, England JD, Philipp MT. (2013) "The Lyme disease spirochete Borrelia burgdorferi induces inflammation and apoptosis in cells from dorsal root ganglia." J Neuroinflammation. 10: 88.
13.) Stavniichuk R, Obrosov AA, Drel VR, Nadler JL, Obrosova IG, Yorek MA. (2013) '12/15-Lipoxygenase inhibition counteracts MAPK phosphorylation in mouse and cell culture models of diabetic peripheral neuropathy.'
14.) Stavniichuk R, Obrosov AA, Drel VR, Nadler JL, Obrosova IG, Yorek MA. (2013) "12/15-Lipoxygenase inhibition counteracts MAPK phosphorylation in mouse and cell culture models of diabetic peripheral neuropathy." J diabetes mellitus. 3.
15.) Peng J, Wang Y, Zhang L, Zhao B, Zhao Z, Chen J, Guo Q, Liu S, Sui X, Xu W, Lu S. (2011) "Human umbilical cord Wharton's jelly-derived mesenchymal stem cells differentiate into a Schwann-cell phenotype and promote neurite outgrowth in vitro." Brain Res Bull. 84: 235-43.
16.) Ahmad Z, Brown CM, Patel AK, Ryan AF, Ongkeko R, Doherty JK. (2010) "Merlin knockdown in human Schwann cells: clues to vestibular schwannoma tumorigenesis." Otol Neurotol. 31: 460-66.
17.) Arima Y, Hayashi H, Kamata K, Goto TM, Sasaki M, Kuramochi A, Saya H. (2010) "Decreased expression of neurofibromin contributes to epithelial-mesenchymal transition in neurofibromatosis type 1." Exp Dermatol. 19: e136-41.
18.) Meyer Zu Horste G, Heidenreich H, Lehmann HC, Ferrone S, Hartung HP, Wiendl H, Kieseier BC. (2010) "Expression of antigen processing and presenting molecules by Schwann cells in inflammatory neuropathies." Glia. 58: 80-92.
19.) Stavniichuk R, Drel VR, Shevalye H, Vareniuk I, Stevens MJ, Nadler JL, Obrosova IG. (2010) "Role of 12/15-lipoxygenase in nitrosative stress and peripheral prediabetic and diabetic neuropathies." Free Radic Biol Med. 49: 1036-45.
20.) Suenaga T, Satoh T, Somboonthum P, Kawaguchi Y, Mori Y, Arase H. (2010) "Myelin-associated glycoprotein mediates membrane fusion and entry of neurotropic herpesviruses." Proc Natl Acad Sci USA. 107: 866-71.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 1704 | HSC cDNA | Human Schwann Cell cDNA |
$453.00
|
