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Skeletal muscle cells, one of the largest cell types in the body, are multinucleated cells formed by the fusion of myoblasts. Skeletal muscle regeneration is a complex process. When skeletal muscle is injured, quiescent resident myoblasts called satellite cells are activated to proliferate, migrate, and differentiate [1]. Various cellular signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinase (MAPK) have been suggested to play an important role in skeletal muscle growth [2]. Insulin-stimulated glucose transport in cultured human skeletal muscle cells is mediated by GLUT4 and heparan sulfate proteoglycan is involved in skeletal muscle differentiation [3]. The fusion of mononucleated cells to form multinucleated myotubes is a central event in skeletal muscle development. Controlling the onset and progression of this process is a complex set of interactions between myoblasts and their environment. Skeletal muscle cell culture is a useful model for studying the process of cell differentiation.
HSkMC from ScienCell Research Laboratories are isolated from human trapezius muscle and erector spinae muscles of the back. HSkMC are cryopreserved at P1 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSkMC are characterized by immunofluorescence with antibodies specific to myosin, actin and actinin. HSkMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSkMC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Skeletal Muscle Cell Medium (SkMCM, Cat. #3501) for the culturing of HSkMC in vitro.
| Catalog No. | 3500 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HSkMC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Villena, J., Brandan, E. (2004) Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle satellite cell proliferation and migration. J Cell Physiol. 198(2):169-78. [2] Morris, R. T., Spangenburg, E. E., Booth, F. W. (2004) Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle. J Appl Physiol. 96(1):398-404. [3] Al-Khalili, L., Chibalin, A. V., Kannisto, K., Zhang, B. B., Permert, J., Holman, G. D., Ehrenborg, E., Ding, V. D., Zierath, J. R., Krook, A. (2004) Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci. 60(5):991-8. |
1.) Zhao, W. & Zhao, S.P. (2015) Different effects of statins on induction of diabetes mellitus: an experimental study Drug Des Devel Ther. 9
2.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
3.) Franzi S, Salajegheh M, Nazareno R, Greenberg SA. (2013) "Type 1 Interferons Inhibit Myotube Formation Independently of Upregulation of Interferon-Stimulated Gene 15." PLoS One. 8: e65362.
4.) Lado-Abeal J, Romero A, Castro-Piedras I, Rodriguez-Perez A, Alvarez-Escudero J. (2010) "Thyroid hormone receptors are down-regulated in skeletal muscle of patients with non-thyroidal illness syndrome secondary to non-septic shock." Eur J Endocrinol. 163: 765-73.
5.) Salajegheh M, Kong SW, Pinkus JL, Walsh RJ, Liao A, Nazareno R, Amato AA, Krastins B, Morehouse C, Higgs BW, Jallal B, Yao Y, Sarracino DA, Parker KC, Greenberg SA. (2010) "Interferon-stimulated gene 15 (ISG15) conjugates proteins in dermatomyositis muscle with perifascicular atrophy." Ann Neurol. 67: 53-63.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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3500| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 3501 | SkMCM | Skeletal Muscle Cell Medium |
$123.00
As low as:
$81.00
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
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| MOLECULAR BIOLOGY | |||||
| 3504 | HSkMC cDNA | Human Skeletal Muscle Cell cDNA |
$411.00
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| 3505 | HSkMC tRNA | Human Skeletal Muscle Cell Total RNA |
$364.00
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| 3506 | HSkMC Lysate | Human Skeletal Muscle Cell Lysate |
$398.00
|
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| 3507 | HSkMC miRNA | Human Skeletal Muscle Cell MicroRNA |
$340.00
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| 3509 | HSkMC gDNA | Human Skeletal Muscle Cell genomic DNA |
$398.00
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