Human Tissue factor/F3 ELISA Kit

Tissue factor (TF), also known as platelet tissue factor, factor III, thrombokinase, or CD142, is a protein present in sub-endothelial tissue and leukocytes that is necessary for the initiation of thrombin formation from the zymogene prothrombin. It is distinct from thromboplastin. The TF gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.

ScienCell's human Tissue Factor ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Tissue factor-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human Tissue Factor captured in strips.