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- Human Tracheal Smooth Muscle Cells
Airway smooth muscle is fundamental for maintaining airway tone. The specialized ultrastructural features and regulatory mechanisms of airway smooth muscle are vital for normal airway function. Previous studies have shown that airway smooth muscle undergoes phenotypic changes during development and in various respiratory disorders. Airway smooth muscle may also mediate the immune response by secreting cytokines and chemokines. Cultured tracheal smooth muscle cells (TSMC) express three p21-activated kinase (PAK) isoforms which may promote TSMC migration by signaling to p38 MAPK. TSMC proliferation is an important component of airway remodeling in asthma and is consequently a target for the development of novel anti-asthma agents.
HTSMC from ScienCell Research Laboratories are isolated from human trachea. HTSMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HTSMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin. HTSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTSMC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing HTSMC in vitro.
| Catalog No. | 3410 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HTSMC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HTSMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Amrani Y, Panettieri RA. (2003) Airway smooth muscle: contraction and beyond. Int J Biochem Cell Biol. 35(3):272-6. [2] Dechert MA, Holder JM, Gerthoffer WT. (2001) p21-activated kinase 1 participates in tracheal smooth muscle cell migration by signaling to p38 Mapk. Am J Physiol Cell Physiol. 281(1):C123-32. |
34101.) Timm DM, Chen J, Sing D, Gage JA, Haisler WL, Neeley SK, Raphael RM, Dehghani M, Rosenblatt KP, Killian TC, Tseng H, Souza GR. (2013) 'A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis.'
2.) Timm DM, Chen J, Sing D, Gage JA, Haisler WL, Neeley SK, Raphael RM, Dehghani M, Rosenblatt KP, Killian TC, Tseng H, Souza GR. (2013) "A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis." Sci rep. 3.
3.) Cheng SE, Lee IT, Lin CC, Kou YR, Yang CM. (2010) "Cigarette smoke particle-phase extract induces HO-1 expression in human tracheal smooth muscle cells: role of the c-Src/NADPH oxidase/MAPK/Nrf2 signaling pathway." Free Radic Biol Med. 48: 1410-22.
4.) Lee CW, Lee IT, Lin CC, Lee HC, Lin WN, Yang CM. (2010) "Activation and induction of cytosolic phospholipase A2 by IL-1beta in human tracheal smooth muscle cells: role of MAPKs/p300 and NF-kappaB." J Cell Biochem. 109: 1045-56.
5.) Cheng SE, Luo SF, Jou MJ, Lin CC, Kou YR, Lee IT, Hsieh HL, Yang CM. (2009) "Cigarette smoke extract induces cytosolic phospholipase A2 expression via NADPH oxidase, MAPKs, AP-1, and NF-kappaB in human tracheal smooth muscle cells." Free Radic Biol Med. 46: 948-60.
6.) Lin WN, Luo SF, Lin CC, Hsiao LD, Yang CM. (2009) "Differential involvement of PKC-dependent MAPKs activation in lipopolysaccharide-induced AP-1 expression in human tracheal smooth muscle cells." Cell Signal. 21: 1385-95.
7.) Luo SF, Chang CC, Lee IT, Lee CW, Lin WN, Lin CC, Yang CM. (2009) "Activation of ROS/NF-kappaB and Ca2+/CaM kinase II are necessary for VCAM-1 induction in IL-1beta-treated human tracheal smooth muscle cells." Toxicol Appl Pharmacol. 237: 8-21.
8.) Yang CM, Lee IT, Lin CC, Yang YL, Luo SF, Kou YR, Hsiao LD. (2009) "Cigarette smoke extract induces COX-2 expression via a PKCalpha/c-Src/EGFR, PDGFR/PI3K/Akt/NF-kappaB pathway and p300 in tracheal smooth muscle cells." Am J Physiol Lung Cell Mol Physiol. 297: L892-902.
9.) Lee CW, Lin CC, Luo SF, Lee HC, Lee IT, Aird WC, Hwang TL, Yang CM. (2008) "Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells." Mol Pharmacol. 73: 1454-64.
10.) Liang KC, Lee CW, Lin WN, Lin CC, Wu CB, Luo SF, Yang CM. (2007) "Interleukin-1beta induces MMP-9 expression via p42/p44 MAPK, p38 MAPK, JNK, and nuclear factor-kappaB signaling pathways in human tracheal smooth muscle cells." J Cell Physiol. 211: 759-70.
11.) Song Y, Qi H, Wu C. (2007) "Effect of 1,25-(OH)2D3 (a vitamin D analogue) on passively sensitized human airway smooth muscle cells." Respirology. 12: 486-94.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1101 | SMCM | Smooth Muscle Cell Medium |
$126.00
As low as:
$86.00
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| MOLECULAR BIOLOGY | |||||
| 3414 | HTSMC cDNA | Human Tracheal Smooth Muscle Cell cDNA |
$453.00
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| 3415 | HTSMC tRNA | Human Tracheal Smooth Muscle Cell Total RNA |
$401.00
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| 3416 | HTSMC Lysate | Human Tracheal Smooth Muscle Cell Lysate |
$438.00
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| 3417 | HTSMC miRNA | Human Tracheal Smooth Muscle Cell MicroRNA |
$374.00
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| 3419 | HTSMC gDNA | Human Tracheal Smooth Muscle Cell genomic DNA |
$438.00
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