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- Human Umbilical Vein Endothelial Cells
Vascular endothelial cells contribute to the maintenance of vascular homeostasis. Vascular endothelial cells produce and secrete activators and inhibitors of the coagulation and fibrinolysis system. In addition, they mediate the adhesion and aggregation of blood platelets. Endothelial cells also release molecules that regulate cell proliferation and control vessel wall tone. Human umbilical vein endothelial cells (HUVEC) are the most commonly used cell type for the study of endothelial cell processes in vitro. HUVEC have a “cobblestone” morphology, show positive staining for vWF/Factor VIII and CD-31, and the ability to take up acetylated low-density lipoprotein. Cells pretreated with IL-1 or TNF-alpha also selectively express E-selectin and VCAM.
HUVEC from ScienCell Research Laboratories are isolated from human umbilical veins. HUVEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HUVEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 and by uptake of DiI-Ac-LDL. HUVEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use endothelial cell medium (ECM, Cat. #1001) for the culturing of HUVEC in vitro.
| Catalog No. | 8000 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HUVEC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Morgan, D. M. L. (1996) Isolation and culture of human umbilical vein endothelial cells. In 'Human Cell Culture Protocols' (Gareth E. Jones, eds.) Humana Press, Totowa, New Jersey, pp.104-109. [2] Newman, P. J. et al., (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247:1219-1222. [3] Bevilaqua, M. P. et al., (1989) Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 243:1160-1165. [4] Osborn, L. et al., (1989) Direct cloning of vascular cell adhesion molecule 1, a cytokine induced endothelial protein that binds to lymphocytes. Cell 59:1203-1211. |
1.) Yan, X., Cao, J., Liang, L., Wang, L., Gao, F., Yang, Z., Duan, J., Chang, T., Deng, S., Liu, Y., Dou, G., Zhang, J., Zheng, Q., Zhang, P. & Han, H. (2016) miR-342-5p Is a Notch Downstream Molecule and Regulates Multiple Angiogenic Pathways Including Notch, Vascular Endothelial Growth Factor and Transforming Growth Factor β Signaling J Am Heart Assoc. 5
2.) He, Y., Luan, Z., Fu, X. & Xu, X. (2016) Overexpression of uncoupling protein 2 inhibits the high glucose-induced apoptosis of human umbilical vein endothelial cells Int J Mol Med. 37
3.) Ye, X., Qian, Y., Wang, Q., Yuan, W., Mo, X., Li, Y., Jiang, Z., Xu, W., Deng, Y., Wan, Y., Fan, X., Wu, X. & Wang, Y. (2016) SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis PLoS One. 11
4.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12
5.) Zhou, W., Tang, W., Sun, Z., Li, Y., Dong, Y., Pei, H., Peng, Y., Wang, J., Shao, T., Jiang, Z., Yi, Z. & Chen, Y.(2016) 'Discovery and Optimization of NSubstituted 2(4pyridinyl)thiazole carboxamides against Tumor Growth through Regulating Angiogenesis Signaling Pathways' Sci Rep. VOL 6
6.) Yan, Y., Jiang, F., Lai, Y., Wang, H., Liu, A., Wang, C., Zhang, Y., Teng, W. & Shan, Z.(2016) 'Effect of Thyrotropin on Osteopontin, Integrin alpha-v-beta-3 and VCAM1 in the Endothelium via Activation of Akt' Int J Mol Sci. VOL 17
7.) Pereira, A.R.S., Mendes, T.F., Ministro, A., Teixeira, M., Filipe, M., Santos, J.M., B?rcia, R.N., GoyriO?Neill, J., Pinto, F., Cruz, P.E., Cruz, H.J. & Santos, S.C.R.(2016) 'Therapeutic angiogenesis induced by human umbilical cord tissuederived mesenchymal stromal cells in a murine model of hindlimb ischemia' Stem Cell Res Ther. VOL 7
8.) Mou, Y., Yue, Z., Wang, X., Li, W., Zhang, H., Wang, Y., Li, R. & Sun, X.(2016) 'OCT4 Remodels the Phenotype and Promotes Angiogenesis of HUVECs by Changing the Gene Expression Profile' Int J Med Sci. VOL 13
9.) Wang, Y., Wang, B., Guerram, M., Sun, L., Shi, W., Tian, C., Zhu, X., Jiang, Z. & Zhang, L. (2015) Deoxypodophyllotoxin suppresses tumor vasculature in HUVECs by promoting cytoskeleton remodeling through LKB1-AMPK dependent Rho A activation Oncotarget. 6
10.) Wang, K., Chen, X., Pan, Y., Cui, Y., Zhou, X., Kong, D. & Zhao, Q. (2015) Enhanced Vascularization in Hybrid PCL/Gelatin Fibrous Scaffolds with Sustained Release of VEGF Biomed Res Int. 2015
11.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
12.) Lau, Y.S., Ling, W.C., Murugan, D., Kwan, C.Y. & Mustafa, M.R. (2015) Endothelium-Dependent Relaxation Effect of Apocynum venetum Leaf Extract via Src/PI3K/Akt Signalling Pathway Nutrients. 7
13.) Jiang, Y.H., Sun, W., Li, W., Hu, H.Z., Zhou, L., Jiang, H.H. & Xu, J.X. (2015) Calycosin-7-O-?-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells BMC Complement Altern Med. 15
14.) Dissanayaka, W.L., Hargreaves, K.M., Jin, L., Samaranayake, L.P. & Zhang, C. (2015) The Interplay of Dental Pulp Stem Cells and Endothelial Cells in an Injectable Peptide Hydrogel on Angiogenesis and Pulp Regeneration In Vivo Tissue Eng Part A. 21
15.) Deng, M., Gu, Y., Liu, Z., Qi, Y., Ma, G.E. & Kang, N. (2015) Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh Stem Cells Int. 2015
16.) Yang, B., Cai, B., Deng, P., Wu, X., Guan, Y., Zhang, B., Cai, W., Schaper, J. & Schaper, W. (2015) Nitric Oxide Increases Arterial Endotheial Permeability through Mediating VE-Cadherin Expression during Arteriogenesis PLoS One. 10
17.) Yang, G., Li, Y., Wu, W., Liu, B., Ni, L., Wang, Z., Miao, S., Wang, L. & Liu, C. (2015) Anti-oxidant effect of heme oxygenase-1 on cigarette smoke-induced vascular injury Mol Med Rep. 12
18.) Ye, Q., Qin, S., Liu, Y., Feng, J., Wu, Q., Qu, W. & Yin, X. (2015) Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular Carcinoma Gastroenterol Res Pract. 2015
19.) Yuan, C., Wang, P., Zhu, L., Dissanayaka, W.L., Green, D.W., Tong, E.H., Jin, L. & Zhang, C. (2015) Coculture of Stem Cells from Apical Papilla and Human Umbilical Vein Endothelial Cell Under Hypoxia Increases the Formation of Three-Dimensional Vessel-Like Structures in Vitro Tissue Eng Part A. 21
20.) Zhang, E., Guo, Q., Gao, H., Xu, R., Teng, S. & Wu, Y. (2015) Metformin and Resveratrol Inhibited High Glucose-Induced Metabolic Memory of Endothelial Senescence through SIRT1/p300/p53/p21 Pathway PLoS One. 10
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8000| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$126.00
As low as:
$83.00
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| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0903 | EndoF | EndoFectagen |
$253.00
|
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| 8248 | BPF | Bovine Plasma Fibronectin |
$124.00
|
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| MOLECULAR BIOLOGY | |||||
| 8004 | HUVEC cDNA | Human Umbilical Vein Endothelial Cell cDNA |
$411.00
|
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| 8005 | HUVEC tRNA | Human Umbilical Vein Endothelial Cell Total RNA |
$364.00
|
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| 8006 | HUVEC Lysate | Human Umbilical Vein Endothelial Cell Lysate |
$398.00
|
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| 8007 | HUVEC miRNA | Human Umbilical Vein Endothelial Cell MicroRNA |
$340.00
|
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| 8009 | HUVEC gDNA | Human Umbilical Vein Endothelial Cell Genomic DNA |
$398.00
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