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- Human Urothelial Cells
Urothelial cells, the cells lining the surface of the urinary bladder, are comprised of a unique cell type with high plasticity and a variety of cell functions. They are the first line of bladder defense and serve as an interface between pathogens. Urothelial cells are equipped with several defense mechanisms to prevent adherence of pathogens and maintain impermeability to urinary solutes. Urothelial cells express both estrogen receptor-alpha and beta, epidermal growth factor receptor and fibroblast growth factor receptor. These receptors play a major role in urothelial cell response to injury and infection. The urothelial cells also release a number of cytokines and other immune system mediators. The ability to culture normal urothelial cells in vitro make it possible to further investigate the immunoregulatory potential of urothelial cells.
HUC from ScienCell Research Laboratories are isolated from the human bladder. HUC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HUC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HUC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Urothelial Cell Medium (UCM, Cat. #4321) for the culturing of HUC in vitro.
Catalog No. | 4320 |
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Country of Manufacture | United States |
Product Code | HUC |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Bjorling, D. E. and Wang, Z. (2001) Estrogen and neuroinflammation. Urology 57:40-46. [2] Daher, A., de Boer, W. I., El-Marjou, A., van der Kwast, T., Abbou, C. C., Thiery, J. P., Radvanyi, F., Chopin, D. K. (2003) Epidermal growth factor receptor regulates normal urothelial regeneration. Lab Invest 83:1333-41. [3]. Bassuk, J. A., Cochrane, K., Mitchell, M. E. (2003) Induction of urothelial cell proliferation by fibroblast growth factor-7 in RAG1-deficient mice. Adv Exp Med Biol 539:623-33. |
1.) Ting, K., Aitken, K.J., Penna, F., Samiei, A.N., Sidler, M., Jiang, J.X., Ibrahim, F., Tolg, C., Delgado-Olguin, P., Rosenblum, N. & Bagli, D.J. (2016) Uropathogenic E.coli (UPEC) Infection Induces Proliferation through Enhancer of Zeste Homologue 2 (EZH2) PLoS One. volume 11
2.) Tylden, G.D., Hirsch, H.H. & Rinaldo, C.H. (2015) Brincidofovir (CMX001) Inhibits BK Polyomavirus Replication in Primary Human Urothelial Cells Antimicrob Agents Chemother. 59
3.) Steins, A., Dik, P., MĀller, W.H., Vervoort, S.J., Reimers, K., Kuhbier, J.W., Vogt, P.M., van Apeldoorn, A.A., Coffer, P.J. & Schepers, K. (2015) In Vitro Evaluation of Spider Silk Meshes as a Potential Biomaterial for Bladder Reconstruction PLoS One. 10
4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
5.) Li, C.F., Wu, W.J., Wu, W.R., Liao, Y.J., Chen, L.R., Huang, C.N., Li, C.C., Li, W.M., Huang, H.Y., Chen, Y.L., Liang, S.S., Chow, N.H. & Shiue, Y.L. (2015) The cAMP responsive element binding protein 1 transactivates epithelial membrane protein 2, a potential tumor suppressor in the urinary bladder urothelial carcinoma Oncotarget. 6
6.) Lee, M.S., Kim, J.H., Lee, J.S., Yun, S.J., Kim, W.J., Ahn, H. & Park, J. (2015) Prognostic Significance of CREB-Binding Protein and CD81 Expression in Primary High Grade Non-Muscle Invasive Bladder Cancer: Identification of Novel Biomarkers for Bladder Cancer Using Antibody Microarray PLoS One. 10
7.) Lu C, Lin M, Chen S, Shen C, Chen L, Hsieh H, Chan MWY, Hsu C. (2013) "The investigation of a traditional Chinese medicine, Guizhi Fuling Wan (GFW) as an intravesical therapeutic agent for urothelial carcinoma of the bladder." BMC Complement Altern Med. 13: 44.
8.) Marentette JO, Hauser PJ, Hurst RE, Klumpp DJ, Rickard A, McHowat J. (2013) "Tryptase activation of immortalized human urothelial cell mitogen-activated protein kinase." PLoS One. 8: e69948.
9.) Noguchi S, Yamada N, Kumazaki M, Yasui Y, Iwasaki J, Naito S, Akao Y. (2013) "socs7, a target gene of microRNA-145, regulates interferon-? induction through STAT3 nuclear translocation in bladder cancer cells." Cell Death Dis. 4: e482.
10.) Lee WY, Savage JR, Zhang J, Jia W, Oottamasathien S, Prestwich GD. (2013) "Prevention of Anti-microbial Peptide LL-37-Induced Apoptosis and ATP Release in the Urinary Bladder by a Modified Glycosaminoglycan." PloS one. 8: e77854.
11.) Tsang M, Chun YW, Im YM, Khang D, Webster TJ. (2011) "Effects of increasing carbon nanofiber density in polyurethane composites for inhibiting bladder cancer cell functions." Tissue Eng Part A. 17: 1879-89.
12.) Bhattacharya A, Tang L, Li Y, Geng F, Paonessa JD, Chen SC, Wong MK, Zhang Y. (2010) "Inhibition of bladder cancer development by allyl isothiocyanate." Carcinogenesis. 31: 281-6.
13.) Koizumi T, Nakatsuji H, Fukawa T, Avirmed S, Fukumori T, Takahashi M, Kanayama H. (2010) "The role of actinin-4 in bladder cancer invasion." Urology. 75: 357-64.
14.) Stone R 2nd, Sabichi AL, Gill J, Lee IL, Adegboyega P, Dai MS, Loganantharaj R, Trutschl M, Cvek U, Clifford JL. (2010) "Identification of genes correlated with early-stage bladder cancer progression." Cancer Prev Res (Phila). 3: 776-86.
15.) Chun YW, Khang D, Haberstroh KM, Webster TJ. (2009) "The role of polymer nanosurface roughness and submicron pores in improving bladder urothelial cell density and inhibiting calcium oxalate stone formation." Nanotechnology. 20: 085104.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
4321 | UCM | Urothelial Cell Medium |
$135.00
As low as:
$90.00
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MOLECULAR BIOLOGY | |||||
4324 | HUC cDNA | Human Urothelial Cell cDNA |
$453.00
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4325 | HUC tRNA | Human Urothelial Cell Total RNA |
$401.00
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4326 | HUC Lysate | Human Urothelial Cell Lysate |
$438.00
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4327 | HUC miRNA | Human Urothelial Cell MicroRNA |
$374.00
|
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4329 | HUC gDNA | Human Urothelial Cell genomic DNA |
$438.00
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