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- Human Villous Trophoblasts
The trophoblast begins at the outer covering of the early blastocyst and provides the route of nourishment between the maternal endometrium and the developing embryo. The trophoblast adhesion to the uterine wall is the requisite first step of implantation and, subsequently, placentation. Human villous tryophoblasts (HVT) covering the villi of the placenta provide the surface for the exchange of oxygen and nutrients with the maternal circulation. They synthesize and release chorionic gonadotropin, placental lactogen and angiogenin and express CXCR4, CCR5 and prolactin gene family. They acquire CCR1 as they differentiate to an invasive phenotype at the villous-anchoring sites. The features of HVT, together with the recent establishment of trophoblast stem cells, make them an ideal genetic platform to study cell differentiation and organogenesis.
HVT from ScienCell Research Laboratories are isolated from human placental villi. HVT are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. HVT are characterized by HCG ELISA. HVT are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HVT are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HVT are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.
Recommended Medium
It is recommended to use Trophoblast Medium (TM, Cat. #7121) for the culturing of HVT in vitro.
| Catalog No. | 7120 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HVT |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Pavlov, N., Hatzi, E., Bassagliam Y., Frendo, J. L., Brion, D. E., Badet, J. (2003) Angiogenin distribution in human term placenta, and expression by cultured trophoblastic cells. Angiogenesis. 6(4):317-30. [2] Maldonado-Estrada, J., Menu, E., Roques, P., Vaslin, B., Dautry-Varsat, A., Barre-Sinoussi, F., Chaouat, G. (2003) Predominant intracellular expression of CXCR4 and CCR5 in purified primary trophoblast cells from first trimester and term human placentae. Am J Reprod Immunol. 50(4):291-301. [3] Wiemers, D. O., Ain, R., Ohboshi, S., Soares, M. J. (2003) Migratory trophoblast cells express a newly identified member of the prolactin gene family. J Endocrinol. 179(3):335-46. [4] Sato, Y., Higuchi, T., Yoshioka, S., Tatsumi, K., Fujiwara, H., Fujii, S. (2003) Trophoblasts acquire a chemokine receptor, CCR1, as they differentiate towards invasive phenotype. Development. 130(22):5519-32. |
71201.) Kudoh, A., Miyakawa, K., Matsunaga, S., Matsushima, Y., Kosugi, I., Kimura, H., Hayakawa, S., Sawasaki, T. & Ryo, A.(2016) 'H11/HSPB8 Restricts HIV2 Vpx to Restore the AntiViral Activity of SAMHD1' Front Microbiol. VOL 7
2.) Issman L, Brenner B, Talmon Y, Aharon A. (2013) "Cryogenic transmission electron microscopy nanostructural study of shed microparticles." PloS one. 8: e83680.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 7121 | TM | Trophoblast Medium |
$122.00
As low as:
$81.00
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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| 0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
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| MOLECULAR BIOLOGY | |||||
| 7124 | HVT cDNA | Human Villous Trophoblast cDNA |
$411.00
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| 7125 | HVT tRNA | Human Villous Trophoblast Total RNA |
$364.00
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| 7126 | HVT Lysate | Human Villous Trophoblast Lysate |
$398.00
|
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| 7127 | HVT miRNA | Human Villous Trophoblast MicroRNA |
$340.00
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| 7129 | HVT gDNA | Human Villous Trophoblast Genomic DNA |
$398.00
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