Mesenchymal Stem Cell Osteogenic Differentiation Medium

Mesenchymal Stem Cell Osteogenic Differentiation Medium (MODM), when used with Mesenchymal Stem Cell Osteogenic Differentiation Supplements (MODS-A, Cat #7532-A and MODS-B, Cat #7532-B) and 25 ml of fetal bovine serum, is a complete medium designed for the optimal osteogenic differentiation of mesenchymal stem cell (MSCs) in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals, and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively promotes osteogenic differentiation of normal human mesenchymal stem cells in vitro.