- Home /
- Mouse Cardiac Myocytes from CD1
Cardiac myocytes are the most physically energetic cells in the body. They are highly specialized high-oxygen-content cells that house a large number of mitochondria. They occupy as much as 75% of the cardiac mass, but constitute only about one third of the total cell number in the heart. Differentiated cardiac myocytes have little capacity to proliferate; however, hypertrophic growth has been shown to respond to alpha1-adrenergic stimuli via the Ras/MEK pathway. All cardiac myocytes are capable of spontaneous rhythmic depolarization and repolarization of their membranes. Contraction of cardiac myocytes is myogenic, which is independent of nervous stimulation. There is a complex network of signals in cardiac myocytes regulating the rhythmic pumping of the heart. Cardiac myocyte hypertrophy and apoptosis have been implicated in the loss of contractile function during heart failure. A better understanding of the cardiac signaling network will help reveal the cellular mechanisms leading to cardiac myocyte death.
MCM from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 CD-1 mouse heart. MCM are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MCM are characterized by immunofluorescence with antibodies specific to sarcomeric alpha-actinin and/or α-smooth muscle actin. MCM are negative for mycoplasma, bacteria, yeast, and fungi. MCM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MCM are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Cardiac Myocyte Medium-serum free (CMM-sf, Cat. #6101) for culturing MCM in vitro.
Catalog No. | M6200 |
---|---|
Country of Manufacture | |
Product Code | MCM |
Size/Quantity | 1 x 10^6 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Bodyak, N., Kang, P. M., Hiromura, M., Sulijoadikusumo, I., Horikoshi, N., Khrapko, K. and Usheva, A. (2002) Gene expression profiling of the aging mouse cardiac myocytes. Nucleic Acids Research 30(17):3788-3794. [2] Tamamori-Adachi, M., Ito, H., Nobori, K., Hayashida, K., Kawauchi, J., Adachi, S., Ikeda, M. A. and Kitajima, S. (2002) Expression of cyclin D1 and CDK4 causes hypertrophic growth of cardiomyocytes in culture: a possible implication for cardiac hypertrophy. Biochem Biophys Res Commun 296(2):274-80. [3] Sambrano, G.R., Fraser, I., Han, H., Ni, Y., O'Connell, T., Yan, Z. and Stull, J. T. (2002) Navigating the signaling network in mouse cardiac myocytes. Nature 420(6916):712-4. |
No Publication available at this time