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- Mouse Embryonic Fibroblasts-mitomycin C treated from CD1
Mouse Embryonic Fibroblasts (MEF) are used to support the growth of undifferentiated mouse or human ES and iPS cells. They provide both a substrate for the ES cells to grow on and secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos and are used at their early passages. These cells have been mitomycin treated to prevent further cell division prior to cryopreservation.
MEF cells from ScienCell Research Laboratories are isolated from E14 mouse embryos. These cells are cryopreserved at P3 after mitomycin C treatment and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. MEF-mt are characterized by immunofluorescent imaging using antibodies to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast and fungi.
Recommended Medium
It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF-mt in vitro.
| Catalog No. | M7540-mt |
|---|---|
| Country of Manufacture | United States |
| Product Code | MEF-mt |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] BRADLEY, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. [2] Nagy et al. (2006) Preparing Mouse Embryo Fibroblasts Cold Spring Harbor Protocols. 2006: pdb.prot 4398. |
Product Sheets
M7540-mt-2 (2 Million Cells per Vial)
M7540-mt-5 (5 Million Cells per Vial)
Certificates of Analysis
For a specific lot number, please search below:
No Publication available at this time
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| M7540-57 | MEF | Mouse Embryonic Fibroblasts from C57BL/6 |
$297.00
|
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| MEDIA | |||||
| 09221 | DMEM | DMEM with High-Glucose, L-Glutamine and Sodium Pyruvate |
$18.00
|
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| M7540 | MEF | Mouse Embryonic Fibroblasts from CD1 |
$130.00
|
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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| 0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
|
