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- Mouse Embryonic Fibroblasts from C57BL/6
Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells [1]. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages [2]. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.
MEF from ScienCell Research Laboratories are isolated from embryonic day 14 C57BL/6 mouse embryos. These cells are cryopreserved at P0 and delivered frozen. Each vial contains 5 x 10^6 cells in 1 ml volume. MEF are characterized by immunofluorescence with antibody specific to fibronectin. MEF are negative for mycoplasma, bacteria, yeast, and fungi. MEF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF in vitro.
| Catalog No. | M7540-57 |
|---|---|
| Country of Manufacture | United States |
| Product Code | MEF |
| Size/Quantity | 5 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] BRADLEY, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. [2] Nagy et al. (2006) Preparing Mouse Embryo Fibroblasts Cold Spring Harbor Protocols. 2006: pdb.prot 4398. |
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M7540-57| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| M7540 | MEF | Mouse Embryonic Fibroblasts from CD1 |
$130.00
|
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| M7540-mt | MEF-mt | Mouse Embryonic Fibroblasts-mitomycin C treated from CD1 |
$25.00
|
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| MEDIA | |||||
| 09221 | DMEM | DMEM with High-Glucose, L-Glutamine and Sodium Pyruvate |
$18.00
|
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| REAGENTS | |||||
| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
