Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls red blood cell production (erythropoiesis). It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. The EPO gene encodes a deduced 193-amino acid propolypeptide hormone that can be found in the kidney and liver. This hormone regulates red blood cell production, plays an important role in the brain's response to neuronal injury, and is involved in the wound healing process. ScienCell's mouse EPO ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse EPO species-specific polyclonal antibodies are precoated onto 8 x 12 divisible strips. The mouse specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added, and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of mouse EPO sample that is captured in strips.