Mouse Hepatic Macrophages (MHMa | ScienCell Research Laboratories

Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy invading pathogens. Mouse Hepatic Macrophages (MHMa), which are also known as Kupffer cells, reside within the lumen of liver sinusoids. MHMa protect the liver by responding to pathogens and metastatic cells, while tolerating harmless self and foreign antigens, which enter via blood flow through the portal vein and hepatic artery. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, fatty liver disease, and liver transplantation. MHMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.

MHMa from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 C57BL/6 mouse liver. MHMa are cryopreserved at P0 and delivered frozen. Each vial contains > 1 x 10^6 cells in 1 ml volume. MHMa are characterized by immunofluorescence with antibody to F4/80. MHMa are negative for mycoplasma, bacteria, yeast, and fungi. MHMa are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories; however, MHMa are not recommended for expanding or long-term cultures since the cells do not proliferate in regular culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing MHMa in vitro.

Catalog No.	M5340-57
Country of Manufacture	United States
Product Code	MHMa
Size/Quantity	Each vial contains > 1 x 10^6 cells in 1 ml volume
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping Info	Dry ice.
References	[1] Liaskou E, Wilson DV, Oo YH. (2012) "Innate immune cells in liver inflammation." Mediators Inflamm. 2012: 949157. [2] Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, Wouters K, Lutjohann D, Gijbels MJ, Febbraio M, Binder CJ, Hofker MH, Shiri-Sverdlov R. (2012) "Internalization of modified lipids by CD36 and SR-A leads to hepatic inflammation and lysosomal cholesterol storage in Kupffer cells." PLoS One. 7: e34378. [3] Tian Y, Jochum W, Georgiev P, Moritz W, Graf R, Clavien PA. (2006) "Kupffer cell-dependent TNF-alpha signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse." Proc Natl Acad Sci U S A. 103: 4598-603. [4] Seki E, de Minicis S, Inokuchi S, Taura K, Miyai K, van Rooijen N, Schwabe RF, Brenner DA. (2009) "CCR2 promotes hepatic fibrosis in mice." Hepatology. 50: 185-97.