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- Mouse Lymphatic Fibroblasts from C57BL/6
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in various organs are intrinsically different [2]. In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion which plays a fundamental role in regulating immune response and homeostasis of tissue fluids [3].
MLF from ScienCell Research Laboratories are isolated from C57BL/6 mouse lymph node. MLF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MLF are characterized by their spindle-shaped morphology and immunofluorescence with antibody specific to fibronectin. MLF are negative for mycoplasma, bacteria, yeast, and fungi. MLF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MLF in vitro.
Catalog No. | M2530-57 |
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Country of Manufacture | United States |
Product Code | MLF |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Gabbiani G, Rungger-Brandle E. (1981) "The fibroblast." In Glynn LE, Handbook of Inflammation, Vol. 3: Tissue Repair and Regeneration (pp 1-50). Amsterdam: Elsevier. [2] Conrad GW, Hart GW, Chen Y. (1977) "Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks." J Cell Sci. 26: 119-37. [3] Roozendaal R, Mebius RE, Kraal G. (2008) "The conduit system of the lymph node." Int Immunol. 20: 1483-7. |
No Publication available at this time
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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M2530 | MLF | Mouse Lymphatic Fibroblasts from CD1 |
$405.00
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MEDIA | |||||
2301 | FM | Fibroblast Medium |
$115.00
As low as:
$70.00
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REAGENTS | |||||
0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
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