The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The brain contains about 1 x 1011 neurons, each being able to contact at least 10,000 other neurons [2].

MN-sc from ScienCell Research Laboratories are isolated from embryonic day 14 C57BL/6 mouse spinal cord. MN-sc are cryopreserved as primary cultures and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MN-sc are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and β-tubulin III. MN-sc are negative for mycoplasma, bacteria, yeast, and fungi. MN-sc are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MN-sc are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.

Recommended Medium

It is recommended to use neuronal medium (NM, Cat. #1521) for culturing MN-sc in vitro.