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- Mouse Renal Proximal Tubular Epithelial Cells
Renal proximal tubular epithelial cells (RPTEpiC) play a crucial role in renal function. They reabsorb nearly all the glucose and amino acids in the glomerular filtrate, while allowing substances of no nutritional value to be excreted into the urine. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. RPTEpiC can produce inflammatory mediators, such as cytokines or chemokines, and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. RPTEpiC express IL-2R alpha and MHC class II antigens during inflammation, after renal transplantation, and during crescentic glomerulonephritis, suggesting that these cells have the capacity to participate in the pathogenesis of immune renal injury. To study the relationship between proximal tubular cells and a variety of renal diseases, the RPTEpiC culture is a useful in vitro model.
MRPTEpiC from ScienCell Research Laboratories are isolated from CD-1 mouse kidney. MRPTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MRPTEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. MRPTEpiC are negative for mycoplasma, bacteria, yeast, and fungi. MRPTEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MRPTEpiC are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing.
Recommended Medium
It is recommended to use Epithelial Cell Medium-animal (EpiCM-a, Cat. #4131) for culturing MRPTEpiC in vitro.
| Catalog No. | M4100 |
|---|---|
| Country of Manufacture | United States |
| Product Code | MRPTEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen, and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] van Kooten, C., van der Linde, X., Woltman, A. M., van Es, L. A. and Daha, M. R. (1999) Synergistic effect of interleukin-1 and CD40L on the activation of human renal tubular epithelial cells. Kidney Int 56(1):41-51. [2]Schmouder, R. L., Strieter, R. M., Wiggins, R. C., Chensue, S. W. and Kunkel, S. L. (1992 ) In vitro and in vivo interleukin-8 production in human renal cortical epithelia. Kidney Int 41(1):191-8. [3] Wuthrich, R. P., Glimcher, L. H., Yui, M. A., Jevnikar, A. M., Dumas, S. E. and Kelley, V. E. (1990) MHC class II, antigen presentation and tumor necrosis factor in renal tubular epithelial cells. Kidney Int 37(2):783-92. |
M41001.) Yu, C.C., Chien, C.T. & Chang, T.C. (2016) M2 macrophage polarization modulates epithelial-mesenchymal transition in cisplatin-induced tubulointerstitial fibrosis BioMedicine. 6
2.) Yu, J., Mao, S., Zhang, Y., Gong, W., Jia, Z., Huang, S. & Zhang, A. (2016) MnTBAP Therapy Attenuates Renal Fibrosis in Mice with 5/6 Nephrectomy Oxid Med Cell Longev. 2016
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| M4100-57 | MRPTEpiC | Mouse Renal Proximal Tubular Epithelial Cells from C57BL/6 |
$489.00
|
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| MEDIA | |||||
| 4131 | EpiCM-a | Epithelial Cell Medium-animal |
$135.00
As low as:
$90.00
|
