The salivary gland produces enzyme-containing saliva to lubricate the oral cavity and assist with digestion. It consists of three pairs of major salivary glands and many minor ones in the oral cavity [1]. Histology studies show that the salivary gland is a compound tubuloalveolar gland, surrounded by connective tissue to further divide the gland into lobes and lobules. The secretory end pieces consist of a spherical mass of cells called acinus. Mouse salivary gland fibroblasts (MSGF) are embryonic mesoderm derived mesenchymal cells and they are responsible for synthesis of extracellular matrix such as type I and/or type III collagen [2]. MSGF are very versatile and provide a useful tool for studying collagenous fibroma and oral neoplasms [3].

MSGF from ScienCell Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse salivary gland. MSGF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MSGF are characterized by immunofluorescence with antibodies specific to fibronectin. MSGF are negative for mycoplasma, bacteria, yeast, and fungi. MSGF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MSGF in vitro.