Custom Order

Customize Your Primers

Stop spending your precious time on primer design and testing.
Let our experts help!


ScienCell Research Laboratories understands that you have unique research needs. We gladly offer custom primer design and kit services. 
Fields marked with * are mandatory.

STEP 1

1. Simply send us a list of your genes-of-interest


Upload your primer list (Word or Excel format only):


OR

2. Assemble your own 96-well qPCR ready kits:

Fill in the 96-well plate below with your genes of interest to get a quote. You may include replicates for statistical analysis, choose from ScienCell existing primer sets, and pick qPCR controls you prefer.

(ScienCell offers 12 housekeeping gene qPCR controls: β-actin, B2M, GAPDH, GUSB, HPRT1, HSP90AB1, LDHA, NONO, PGK1, PPIH, RPLP0, and TFRC. In addition, Genomic DNA Control (GDC), Positive PCR Control (PPC), and NO Template Control (NTC) are highly recommended for qPCR assays.)

 

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STEP 2

Description of the product format and quantity*
e.g. primers in 96-well plates or in tubes, plate types, quantity (number of reactions), and any specific requirements.

STEP 3

Customer Information:

We will deliver qPCR ready kits and primer products in a timely manner to support your research, and make sure that:

(i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO)
(ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated and
(iii) only one gene is amplified


In addition, all primer sets will be validated by qPCR with melt curve analysis, and gel electrophoresis.

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