Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.
ScienCell's Relative Human Telomere Length Quantification qPCR Assay Kit (RHTLQ) is designed to directly compare the average telomere length of the samples. The telomere primer set recognizes and amplifies telomere sequences. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on human chromosome 17, and serves as reference for data normalization. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.
Kit Components:
| Cat # |
Component |
Quantity |
Storage |
| MB6018a-1 |
2X GoldNStart TaqGreen qPCR master mix, 1 mL |
2 vials |
-20°C |
| 8908a |
Telomere primer set, lyophilized |
1 vial |
-20°C |
| 8908b |
Single copy reference (SCR) primer set, lyophilized |
1 vial |
-20°C |
| 8908c |
Nuclease-free H2O |
4 mL |
4 °C |
Product Sheets
8908
Certificates of Analysis
For a specific lot number, please search below:
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I find the assays to be very easy to execute Review by Carla G.
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| Overall quality of the product |
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| Technical resources |
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I find the assays to be very easy to execute. The protocols for setting up the assay and computing the data is very straightforward and user friendly. Most importantly the technical support that I have received as I have tested the assays has been phenomenal. (Posted on 4/10/2018)
1.) Seki, Y., Aczel, D., Torma, F., Jokai, M., Boros, A., Suzuki, K., Higuchi, M., Tanisawa, K., Boldogh, I., Horvath, S., & Radak, Z. (2023). No Strong Association among epigenetic modifications by DNA methylation, telomere length, and physical fitness in Biological Aging. Biogerontology. https://doi.org/10.1007/s10522-022-10011-0
2.) Martin LF, Richardson LS, da Silva MG, Sheller- Miller S, Menon R. (2019) Dexamethasone Induces Primary Amnion Epithelial Cell Senescence through Telomere-P21 Associated Pathway. Biology of Reproduction.
3.) Bruno S, Sanchez MBH, Pasquino C, Tapparo M, Cedrino M, Tetta C, Camussi G. (2019) "Human Liver-Derived Stem Cells Improve Fibrosis and Inflammation Associated with Nonalcoholic Steatohepatitis." Stem Cells International. 2019
4.) Wysoczanska B, Dratwa M, Gebura K, Mizgala J, Mazur G, Wrobel T, Bogunia-Kubik K. (2019) "Variability Within the Human TERT Gene, Telomere Length and Predisposition to Chronic Lymphocytic Leukemia." OncoTargets and Therapy. 12:4309-4320
5.) Chung SS, Dutta P, Chard N, Wu Y, Chen QH, Chen G, Vadgama J. (2019) "A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-kB inactivation in colorectal cancer cells." Oncotarget. 10(44):4516-4531
6.) Babu H, Ambikan AT, Gabriel EE, Akusjarvi SS, Mupanni NR, Sperk M, Cheedarla N, Palaniapan AN, Sridhar R, Sundaraj V, Tripathy SP, Nowak P, Hanna LE, Neogi U. (2018) Systemic inflammation following long-term successful antiretroviral therapy in people living with HIV (PLHIV). bioRxiv preprint.
7.) Iezzi I, Cerqueni G, Licini c, Lucarini G, Belmonte M. (2018) Dental pulp stem cells senescence and regenerative potential relationship. Journal of Cellular Physiology. 234(5):7186-7197
8.) Lazzarini R, Nicolai M, Pirani V, Mariorri C, DiPrimio R. (2018) Effects of senescent secretory phenotype acquisition on human retinal pigment epithelial stem cells. Aging. 10(11):3173-3184
9.) Shirato K, Takanari J, Koda T, Sakurai T, Ogasawara J, Ohno H, Kizaki T. (2018) A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study. Environmental Health and Preventative Medicine. 23:40
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
