Rat | ScienCell Research Laboratories

Meningeal cells surrounding the brain participate actively in the normal development of the central nervous system. They play important roles in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, laminating the cerebellar cortex. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex and dentate gyrus. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex in adult rat spinal cord lesion, promote axonal regrowth. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells.

RMC from ScienCell Research Laboratories are isolated from CD® IGS rat leptomeningi. RMC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RMC are characterized by immunofluorescence; they are positive for fibronectin and negative for GFAP. RMC are negative for mycoplasma, bacteria, yeast and fungi. RMC are guaranteed to further expand for 5 population doublings under the conditions specified by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Meningeal Cell Medium (MenCM, Cat. #1401) for the culturing of RMC in vitro.

Catalog No.	R1400
Country of Manufacture	United States
Product Code	RMC
Size/Quantity	5 x 10^5 cells/vial
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping Info	Dry ice.
References	[1] Hartmann, D., Sievers, J. Pehlemann, F. W. and Berry, M. (1992) Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61. [2] Franzen, R., Martin, D., Daloze, A., Moonen, G. and Schoenen, J. (1999) Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556. [3] Hartmann, D., Schulze, M. and Sievers, J. (1998) Meningeal cells stimulate and direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.