Mouse | ScienCell Research Laboratories

Meningeal cells surround the brain and actively participate in the development of the central nervous system. They play an important role in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, and laminating the cerebellar cortex. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerebellar cortex and dentate gyrus. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex, in adult rat spinal cord lesions promotes axonal regrowth. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells.

MMC from ScienCell Research Laboratories are isolated from C57BL/6 mouse leptomeningi. MMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MMC are characterized by immunofluorescence; they are positive for fibronectin and negative for GFAP. MMC are negative for mycoplasma, bacteria, yeast, and fungi. MMC are guaranteed to further expand for 5 population doublings under the conditions specified by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Meningeal Cell Medium (MenCM, Cat. #1401) for culturing MMC in vitro.

Catalog No.	M1400-57
Country of Manufacture	United States
Product Code	MMC
Size/Quantity	5 x 10^5 cells/vial
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keepthe cells in liquid nitrogen until cell culture is needed for experiments.
Shipping Info	Dry ice.
References	[1] Hartmann, D., Sievers, J. Pehlemann, F. W. and Berry, M. (1992) Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61. [2] Franzen, R., Martin, D., Daloze, A., Moonen, G. and Schoenen, J. (1999) Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556. [3] Hartmann, D., Schulze, M. and Sievers, J. (1998) Meningeal cells stimulate and direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.