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- Human Thymic Fibroblasts
Fibroblasts have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix (ECM) which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. A previous study demonstrated that thymic fibroblasts are derived from the embryonic neural crest, which first surrounds the thymus as mesenchyme and later migrates into the thymic epithelium to form a network of fibroblasts. Thymic fibroblasts and the associated ECM have been
shown to be responsible for normal thymus morphogenesis and lymphopoiesis.
HTyF from ScienCell Research Laboratories are isolated from human fetal thymus. HTyF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HTyF are characterized by immunofluorescence with antibody specific to fibronectin. HTyF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HTyF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HTyF in vitro.
Catalog No. | 3930 |
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Country of Manufacture | United States |
Product Code | HTyF |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Gabbiani G, Rungger-Brandle E. (1981) "The fibroblast." In Glynn LE, Handbook of Inflammation, Vol. 3: Tissue Repair and Regeneration (pp 1-50). Amsterdam: Elsevier. [2] Conrad GW, Hart GW, Chen Y. (1977) "Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks." J Cell Sci. 26: 119-37. [3] Le Lievre CS, Le Douarin NM. (1975) . "Mesenchymal derivatives of the neural crest: analysis of chimaeric quail and chick embryos." J Embryol Exp Morphol. 34: 125-54. [4] Suniara RK, Jenkinson EJ, Owen JJ. (2000) "An essential role for thymic mesenchyme in early T cell development." J Exp Med. 191: 1051-6. |
No Publication available at this time
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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3934 | HTyF cDNA | Human Thymic Fibroblast cDNA |
$453.00
|
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3935 | HTyFtRNA | Human Thymic Fibroblast Total RNA |
$401.00
|
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3936 | HTyF lysate | Human Thymic Fibroblast Lysate |
$438.00
|
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3937 | HTyF miRNA | Human Thymic Fibroblast MicroRNA |
$374.00
|
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3939 | HTyF gDNA | Human Thymic Fibroblast Genomic DNA |
$438.00
|