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- Human Brain Vascular Smooth Muscle Cells
Vascular smooth muscle cells (VSMC) significantly contribute to the development of vascular disease. The ability of VSMC to switch to a proliferative phenotype in response to environmental stimuli is a major factor in the progression of vascular disease. VSMC express the cell adhesion molecules ICAM-1 and VCAM-1 and studies have suggested that these molecules may promote VSMC proliferation, VSMC differentiation, and vascular inflammation. Human VSMC cultures
play a critical role in the continuous advancement of therapies for vascular disease.
HBVSMC from ScienCell Research Laboratories are isolated from human brain. HBVSMC are cryopreserved at passage one after purification and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HBVSMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin. HBVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HBVSMC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing HBVSMC in vitro.
| Catalog No. | 1100 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HBVSMC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HBVSMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication of smooth muscle cells in vascular disease. Circ. Res. 58:427-444. [2] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S. B. (1999) Cellular adhesion molecules on vascular smooth muscle cells. Cardiovasc. Res. 41:395-401. |
11001.) Reynolds, M.R., Singh, I., Azad, T.D., Holmes, B.B., Verghese, P.B., Dietrich, H.H., Diamond, M., Bu, G., Han, B.H. & Zipfel, G.J. (2016) Heparan sulfate proteoglycans mediate A?-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells Mol Neurodegener. 11
2.) Kikkawa Y, Matsuo S, Kurogi R, Nakamizo A, Mizoguchi M, Sasaki T. (2014) 'Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits.'
3.) Shi X, Fu Y, Liao D, Chen Y, Liu J. (2014) 'Alterations of Voltage-Dependent Calcium Channel Currents in Basilar Artery Smooth Muscle Cells at Early Stage of Subarachnoid Hemorrhage in a Rabbit Model.'
4.) Kikkawa Y, Matsuo S, Kurogi R, Nakamizo A, Mizoguchi M, Sasaki T. (2014) "Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits." Biomed res int. 2014: 836397.
5.) Shi X, Fu Y, Liao D, Chen Y, Liu J. (2014) "Alterations of Voltage-Dependent Calcium Channel Currents in Basilar Artery Smooth Muscle Cells at Early Stage of Subarachnoid Hemorrhage in a Rabbit Model." PloS one. 9: e84129.
6.) Cucullo L, Hossain M, Tierney M, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: cerebrovascular physiology in a box." BMC Neurosci. 14: 18.
7.) Meng H, Zhang X, Lee SJ, Wang MM. (2013) "Von Willebrand Factor Inhibits Mature Smooth Muscle Gene Expression through Impairment of Notch Signaling." PLoS One. 8: e75808.
8.) Miners JS, Kehoe P, Love S. (2011). "Neprilysin protects against cerebral amyloid angiopathy and Abeta-induced degeneration of cerebrovascular smooth muscle cells." Brain Patholology. 21: 594-605
9.) Fossati S, Cam J, Meyerson J, Mezhericher E, Romero IA, Couraud PO, Weksler BB, Ghiso J, Rostagno A. (2010) "Differential activation of mitochondrial apoptotic pathways by vasculotropic amyloid-beta variants in cells composing the cerebral vessel walls." FASEB J. 24: 229-41.
10.) Lefterov I, Fitz NF, Cronican AA, Fogg A, Lefterov P, Kodali R, Wetzel R, Koldamova R. (2010) "Apolipoprotein A-I deficiency increases cerebral amyloid angiopathy and cognitive deficits in APP/PS1DeltaE9 mice." J Biol Chem 285: 36945-57.
11.) Kanekiyo T, Bu G. (2009) "Receptor-associated protein interacts with amyloid-beta peptide and promotes its cellular uptake." J Biol Chem. 284: 33352-59.
12.) Wang J, Tuo H, Wang R, Li W, Feng Z, Chen J, Wang D. (2008) "The expression of human brain vascular smooth muscle cell AT receptor after the UL83 gene of HCMV inhibition by small interfering RNAs." Neurol Res. 30: 903-09.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1101 | SMCM | Smooth Muscle Cell Medium |
$126.00
As low as:
$86.00
|
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| MOLECULAR BIOLOGY | |||||
| 1104 | HBVSMC cDNA | Human Brain Vascular Smooth Muscle Cell cDNA |
$453.00
|
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| 1105 | HBVSMC tRNA | Human Brain Vascular Smooth Muscle Cell Total RNA |
$401.00
|
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| 1106 | HBVSMC Lysate | Human Brain Vascular Smooth Muscle Cell Lysate |
$438.00
|
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| 1107 | HBVSMC miRNA | Human Brain Vascular Smooth Muscle Cell MicroRNA |
$374.00
|
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| 1109 | HBVSMC gDNA | Human Brain Vascular Smooth Muscle Cell Genomic DNA |
$438.00
|
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