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- Human Pulmonary Alveolar Epithelial Cells
Pulmonary alveolar epithelial cells (PAEpiC), comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal surface area. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the surface area, produce, secrete, and recycle pulmonary surfactant. Type II cells contain Na+-, K+-ATPase and amiloride-sensitive epithelial Na+ channels. The currently accepted hypothesis is that Type II cells maintain pulmonary fluid homeostasis by regulating active Na+ transport in the lungs, whereas Type I cells are "inert" cells that provide solely a barrier function, rather than having active functions. Recent studies indicate
that Type I cells are also important in regulating ion and fluid transport.
HPAEpiC from ScienCell Research Laboratories are isolated from human lung tissue. HPAEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HPAEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HPAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HPAEpiC are not recommended for expanding or long-term cultures since the cells differentiate to become type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.
Recommended Medium
It is recommended to use Alveolar Epithelial Cell Medium (AEpiCM, Cat. #3201) for culturing HPAEpiC in vitro.
| Catalog No. | 3200 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HPAEpiC |
| Size/Quantity | 1 x 10^6 cells |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Crapo, J. D., Young, S. L., Fram, E. K., Pinkerton, K. E., Barry, B. E., & Crapo, R. O. (1983) Morphometric characteristics of cells in the alveolar region of mammalian lungs. Am. Rev. Respir. Dis. 128:S42-S46. [2] Wright, J. R. & Dobbs, L. G. (1991) Regulation of pulmonary surfactant secretion and clearance. Annu. Rev. Physiol. 53:395-414. [3] Johnson, M. D., Widdicombe, J. H., Allen, L., Barbry, P. and Dobbs, L. G. (2002) Alveolar epithelial type I cells contain transport proteins and transport sodium, supporting an active role for type I cells in regulation of lung liquid homeostasis Proc. Natl. Acad. Sci. USA. 99(4):1966-1971. |
32001.) Li, Q., Deng, M.-S., Wang, R.-T., Luo, H., Luo, Y.-Y., Zhang, D.-D., Chen, K.-J., Cao, X.-F., Yang, G.-M., Zhao, T.-M., Xu, B., Xu, C.-X., & Wang, J.-M. (2023). PD-L1 upregulation promotes drug-induced pulmonary fibrosis by inhibiting vimentin degradation. Pharmacological Research, 187, 106636. https://doi.org/10.1016/j.phrs.2022.106636
2.) Akimoto, M., Hayashi, J.I., Nakae, S., Saito, H. & Takenaga, K. (2016) Interleukin-33 enhances programmed oncosis of ST2L-positive low-metastatic cells in the tumour microenvironment of lung cancer Cell Death Dis. 7
3.) Ilia Atanelishvili ,
Yuichiro Shirai ,
Tanjina Akter,
Atsushi Noguchi,
Kurt T. Ash,
Suniti Misra,
Sibnath Ghatak,
Richard M. Silver,
Galina S. Bogatkevich
(2016) "D1398G Variant of MET Is Associated with Impaired Signaling of Hepatocyte Growth Factor in Alveolar Epithelial Cells and Lung Fibroblasts" PLoS ONE 11(9): e0162357.
4.) Cao J, Wang D, Xu F, Gong Y, Wang H, Song Z, Li D, Zhang H, Li D, Zhang L, Xia Y, Xu H, Lai X, Lin S, Zhang X, Ren G, Dai Y, Yin Y. (2014) 'Activation of IL?27 signalling promotes development of postinfluenza pneumococcal pneumonia.'
5.) Cao J, Wang D, Xu F, Gong Y, Wang H, Song Z, Li D, Zhang H, Li D, Zhang L, Xia Y, Xu H, Lai X, Lin S, Zhang X, Ren G, Dai Y, Yin Y. (2014) "Activation of IL?27 signalling promotes development of postinfluenza pneumococcal pneumonia." EMBO mol med. 6: 120-140.
6.) Lee IT, Lin CC, Cheng SE, Hsiao LD, Hsiao YC, Yang CM. (2013) "TNF-α Induces Cytosolic Phospholipase A2 Expression in Human Lung Epithelial Cells via JNK1/2- and p38 MAPK-Dependent AP-1 Activation." 8: e72783.
7.) Eckle T, Brodsky K, Bonney M, Packard T, Han J, Borchers CH, Mariani TJ, Kominsky DJ, Mittelbronn M, Eltzschig HK. (2013) "HIF1A Reduces Acute Lung Injury by Optimizing Carbohydrate Metabolism in the Alveolar Epithelium." PLoS Biol. 11: e1001665.
8.) Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. (2013) 'Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors.'
9.) Chang RS, Wang YC, Kao ST. (2013) 'Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin.'
10.) Chang RS, Wang YC, Kao ST. (2013) "Soluble toll-like receptor 4 reversed attenuating effect of Chinese herbal Xiao-Qing-Long-Tang on allergen induced nerve growth factor and thymic stromal lymphopoietin." Expther med. 6: 1199-1207.
11.) Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. (2013) "Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors." Diagnpathol.8: 201.
12.) Kuo YB, Chang CA, Wu YK, Hsieh MJ, Tsai CH, Chen KT, Chen CY, Chan EC. (2010) "Identification and clinical association of anti-cytokeratin 18 autoantibody in COPD." Immunol Lett. 128: 131-6.
13.) Lin CC, Kuo CT, Cheng CY, Wu CY, Lee CW, Hsieh HL, Lee IT, Yang CM. (2009) "IL-1 beta promotes A549 cell migration via MAPKs/AP-1- and NF-kappaB-dependent matrix metalloproteinase-9 expression." Cell Signal. 21: 1652-62.
14.) Malizia AP, Egan JJ, Doran PP. (2009) "IL-4 increases CD21-dependent infection of pulmonary alveolar epithelial type II cells by EBV." Mol Immunol. 46: 1905-10.
15.) Lin CC, Tseng HW, Hsieh HL, Lee CW, Wu CY, Cheng CY, Yang CM. (2008) "Tumor necrosis factor-alpha induces MMP-9 expression via p42/p44 MAPK, JNK, and nuclear factor-kappaB in A549 cells." Toxicol Appl Pharmacol. 229: 386-98.
16.) Gentry M, Taormina J, Pyles RB, Yeager L, Kirtley M, Popov VL, Klimpel G, Eaves-Pyles T. (2007) "Role of primary human alveolar epithelial cells in host defense against Francisella tularensis infection." Infect Immun. 75: 3969-78.
17.) Sims AC, Baric RS, Yount B, Burkett SE, Collins PL, Pickles RJ. (2005) "Severe acute respiratory syndrome coronavirus infection of human ciliated airway epithelia: role of ciliated cells in viral spread in the conducting airways of the lungs." J Virol. 79: 15511-24.
18.) Tsuji T, Aoshiba K, Nagai A. (2004) "Cigarette Smoke Induces Senescence in Alveolar Epithelial Cells American." Am J Respir Cell Mol Biol. 31: 643-9.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 3201 | AEpiCM | Alveolar Epithelial Cell Medium |
$135.00
As low as:
$90.00
|
||
| MOLECULAR BIOLOGY | |||||
| 3204 | HPAEpiC cDNA | Human Pulmonary Alveolar Epithelial Cell cDNA |
$453.00
|
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| 3205 | HPAEpiC tRNA | Human Pulmonary Alveolar Epithelial Cell Total RNA |
$401.00
|
||
| 3206 | HPAEpiC Lysate | Human Pulmonary Alveolar Epithelial Cell Lysate |
$438.00
|
||
| 3207 | HPAEpiC miRNA | Human Pulmonary Alveolar Epithelial Cell MicroRNA |
$374.00
|
||
| 3209 | HPAEpiC gDNA | Human Pulmonary Alveolar Epithelial Cell genomic DNA |
$438.00
|
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