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- Human Pulmonary Fibroblasts
The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, such as long branching processes and gap junctions. HPF main function is to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial for effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts can result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension.
HPF from ScienCell Research Laboratories are isolated from human lung tissue. HPF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HPF are characterized by immunofluorescence with antibody specific to fibronectin. HPF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HPF in vitro.
| Catalog No. | 3300 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HPF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Kuwano K, Hagimoto N, Hara N. (2001) Molecular mechanisms of pulmonary fibrosis and current treatment. Curr Mol Med 1(5):551-73. [2] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86. |
33001.) Sarker AH, Chatterjee A, Williams M, Lin S, Havel C, Jacob III P, Boldogh I, Hazra TK, Talbot P, Hang B. (2014) 'NEIL2 Protects against Oxidative DNA Damage Induced by Sidestream Smoke in Human Cells.'
2.) Sarker AH, Chatterjee A, Williams M, Lin S, Havel C, Jacob III P, Boldogh I, Hazra TK, Talbot P, Hang B. (2014) "NEIL2 Protects against Oxidative DNA Damage Induced by Sidestream Smoke in Human Cells." PloS one. 9: e90261.
3.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.
4.) Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX. (2010) "Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension." Am J Physiol Cell Physiol. 298: C1217-25.
5.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 3304 | HPF cDNA | Human Pulmonary Fibroblast cDNA |
$453.00
|
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| 3305 | HPF tRNA | Human Pulmonary Fibroblast Total RNA |
$401.00
|
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| 3306 | HPF Lysate | Human Pulmonary Fibroblast Lysate |
$438.00
|
||
| 3307 | HPF miRNA | Human Pulmonary Fibroblast MicroRNA |
$374.00
|
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| 3309 | HPF gDNA | Human Pulmonary Fibroblast genomic DNA |
$438.00
|
