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Renal mesangial cells (RMC) are perivascular cells located within the central portion of the glomerular tuft between capillary loops. Mesangial cells have a variety of functions including synthesis and assembly of the mesangial matrix, endocytosis and processing of plasma macromolecules, and control of glomerular hemodynamics via mesangial cell contraction or release of vasoactive hormones. Mesangial cells also synthesize, assemble and control turnover of the mesangial matrix. Matrix overproduction of mesangial cells has been observed in various glomerular diseases, such as IgA nephropathy, mesangioproliferative glomerulonephritis, lupus nephritis, glomerulosclerosis and diabetic nephropathy. Such critical involvements suggest renal mesangial cells are an ideal model for studying mesangial injury and glomerular functions under both physiological and pathophysiological conditions.
HRMC from ScienCell Research Laboratories are isolated from human renal tissue. HRMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HRMC are characterized by immunofluorescence with antibodies specific to fibronectin, Thy-1, and/or smooth muscle actin. HRMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRMC are guaranteed to further expand for 15 population doublings under the conditions specified by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesangial Cell Medium (MsCM, Cat. #4201) for the culturing of HRMC in vitro.
| Catalog No. | 4200 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HRMC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Rodriguez-Barbero, A., L'Azou, B., Cambar, J. and Lopez-Novoa, J. M. (2000) Potential use of isolated glomeruli and cultured mesangial cells as in vitro models to assess nephrotoxicity. Cell Biol Toxicol 16(3):145-53 [2] Stockand, J. D. and Sansom, S. C. (1977) Regulation of filtration rate by glomerular mesangial cells in health and diabetic renal disease. Am J Kidney Dis 29(6): 971-81. [3] Grond, J. and Weening, J. J. (1990) Mesangial cell injury, glomerulosclerosis, and therapeutic intervention. Contrib Nephrol 81: 229-39. |
42001.) Jia, Y., Guan, M., Zheng, Z., Zhang, Q., Tang, C., Xu, W., Xiao, Z., Wang, L. & Xue, Y. (2016) miRNAs in Urine Extracellular Vesicles as Predictors of Early-Stage Diabetic Nephropathy Journal of Diabetes Research. 2016
2.) Dai, Q., Liu, J., Du, Y., Hao, X., Ying, J., Tan, Y., He, L., Wang, W. & Chen, N. (2016) Histone deacetylase inhibitors attenuate P-aIgA1-induced cell proliferation and extracellular matrix synthesis in human renal mesangial cells in vitro Acta Pharmacol Sin. 37
3.) Ghada AK and J Golbahar. (2013) ?High Glucose-Induced Oxidative Stress Increases the Copy Number of Mitochondrial DNA in Human Mesangial Cells.? BioMed Research International. 2013: 1155-63
4.) Kang MK, Lim SS, Lee JY, Yeo KM, Kang YH. (2013) "Anthocyanin-Rich Purple Corn Extract Inhibit Diabetes-Associated Glomerular Angiogenesis." PloS one. 8: e79823.
5.) Li J, Lim SS, Lee ES, Gong JH, Shin D, Kang IJ, Kang YH. (2011) "Isoangustone A suppresses mesangial fibrosis and inflammation in human renal mesangial cells." Exp Biol Med (Maywood). 236: 435-44.
6.) Wu F, Zhang W, Li L, Zheng F, Shao X, Zhou J, Li H. (2011) "Inhibitory effects of honokiol on lipopolysaccharide-induced cellular responses and signaling events in human renal mesangial cells." Eur J Pharmacol. 654: 117-21.
7.) Huang P, Zhang Y, Jiang T, Zeng W, Zhang N. (2010) "Role of aldose reductase in the high glucose induced expression of fibronectin in human mesangial cells." Mol Biol Rep. 37: 3017-21.
8.) Li J, Kang SW, Kim JL, Sung HY, Kwun IS, Kang YH. (2010) "Isoliquiritigenin entails blockade of TGF-beta1-SMAD signaling for retarding high glucose-induced mesangial matrix accumulation." J Agric Food Chem. 58: 3205-12.
9.) Jiang T, Qu JJ, Nishinaka T, Zhang N. (2008) "Transcription factor AP-1 regulates TGF-beta(1)-induced expression of aldose reductase in cultured human mesangial cells." Nephrology (Carlton). 13: 212-7.
10.) Mathew JT, Patni H, Chaudhary AN, Liang W, Gupta A, Chander PN, Ding G, Singhal PC. (2008) "Aldosterone induces mesangial cell apoptosis both in vivo and in vitro." Am J Physiol Renal Physiol. 295: F73-81.
11.) Drel VR, Pacher P, Stevens MJ, Obrosova IG. (2006) "Aldose reductase inhibition counteracts nitrosative stress and poly(ADP-ribose) polymerase activation in diabetic rat kidney and high-glucose-exposed human mesangial cells." Free Radic Biol Med. 40: 1454-65.
12.) Li Y, Wen X, Spataro BC, Hu K, Dai C, Liu Y. (2006) "Hepatocyte growth factor is a downstream effector that mediates the antifibrotic action of peroxisome proliferator-activated receptor-gamma agonists." J Am Soc Nephrol. 17: 54-65.
13.) Wen X, Li Y, Hu K, Dai C, Liu Y. (2005) "Hepatocyte growth factor receptor signaling mediates the anti-fibrotic action of 9-cis-retinoic acid in glomerular mesangial cells." Am J Pathol. 167: 947-57.
14.) Dai C, Liu Y. (2004) "Hepatocyte growth factor antagonizes the profibrotic action of TGF-beta1 in mesangial cells by stabilizing Smad transcriptional corepressor TGIF." J Am Soc Nephrol. 15: 1402-12.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4201 | MCM | Mesangial Cell Medium |
$135.00
As low as:
$90.00
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| MOLECULAR BIOLOGY | |||||
| 4204 | HRMC cDNA | Human Renal Mesangial Cell cDNA |
$453.00
|
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| 4205 | HRMC tRNA | Human Renal Mesangial Cell Total RNA |
$401.00
|
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| 4206 | HRMC Lysate | Human Renal Mesangial Cell Lysate |
$438.00
|
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| 4207 | HRMC miRNA | Human Renal Mesangial Cell MicroRNA |
$374.00
|
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| 4209 | HRMC gDNA | Human Renal Mesangial Cell genomic DNA |
$438.00
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