Human Gastric Fibroblasts (HGF) Catalog #2830 | ScienCell Research Laboratories

Gastric fibroblasts (GF), which are located in the stomach, are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Studies in vitro and in vivo show that GF can regulate epithelial regeneration by influencing epithelial cell proliferation and differentiation. In addition to their role in wound healing, human GF (HGF) have been shown to be key contributors in the development and progression of gastric carcinoma. HGF are a useful model for examining the underlying mechanisms that cause gastric carcinoma and may provide novel insights for developing new cancer drug therapies.

HGF from ScienCell Research Laboratories are isolated from human stomach tissue. HGF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HGF are characterized by immunofluorescence with antibody specific to fibronectin. HGF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research
Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HGF in vitro.

Catalog No.	2830
Country of Manufacture	United States
Product Code	HGF
Size/Quantity	5 x 10^5 cells/vial
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping Info	Dry ice.
References	[1] Gabbiani G, Rungger-Brandle E. (1981). "The Fibroblast." In Glynn LE, Handbook of Inflammation, Vol. 3: Tissue Repair and Regeneration (pp.1-50). Amsterdam: Elsevier. [2] Piazuelo E, Lana A, Jimenez P, Garcia-Gonzalez A, Esteva F. (1998) "In vitro wound repair by human gastric fibroblast: implications for ulcer healing." Dig Dis Sci. 43(6): 1230-1240. [3] Nakazawa K, Yashiro M, Hirakawa K. (2003) "Keratinocyte growth factor produced by gastric fibroblasts specifically stimulates proliferation of cancer cells from scirrhous gastric carcinoma." Cancer Res. 63(24): 8848-8852.