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- Human Bone Marrow-derived Mesenchymal Stem Cells
Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of MSC in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. MSC have the capability for renewal and differentiation into various lineages of mesenchymal tissues. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold to generate appropriate tissue constructs.
HMSC-bm from ScienCell Research Laboratories are isolated from human bone marrow. HMSC-bm are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMSC-bm are characterized by immunofluorescence with antibodies specific to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and Alizarin Red staining after osteogenic differentiation. HMSC-bm are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMSC-bm are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing HMSC-bm in vitro.
| Catalog No. | 7500 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HMSC-bm |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | 1. Kassem, M. Mesenchymal stem cells: biological characteristics and potential clinical applications. 2004. Cloning Stem Cells. 6(4):369-74. 2. Barry, F. P., and J. M. Murphy. Mesenchymal stem cells: clinical applications and biological characterization. 2004. Int J Biochem Cell Biol. 36(4):568-84. |
75001.) Nguyen, H. T., Ho, T.-L., Pratomo, A., Ilsan, N. A., Huang, T.-wen, Chen, C.-H., & Chuang, E.-Y. (2021). Enzymatically triggered graphene oxide released from multifunctional carriers boosts anti-pathogenic properties for promising wound-healing applications. Materials Science and Engineering: C, 128, 112265. https://doi.org/10.1016/j.msec.2021.112265
2.) Li, G.C., Zhang, H.W., Zhao, Q.C., Sun, L., Yang, J.J., Hong, L., Feng, F. & Cai, L. (2016) Mesenchymal stem cells promote tumor angiogenesis via the action of transforming growth factor ?1 Oncol Lett. 11
3.) Lee, M.S., Yuan, H., Jeon, H., Zhu, Y., Yoo, S., Shi, S., Krueger, B., Renne, R., Lu, C., Jung, J.U. & c (2016) Human Mesenchymal Stem Cells of Diverse Origins Support Persistent Infection with Kaposi?s Sarcoma-Associated Herpesvirus and Manifest Distinct Angiogenic, Invasive, and Transforming Phenotypes mBio. 7
4.) Li, W., Zheng, Y., Zhao, X., Ge, Y., Chen, T., Liu, Y. & Zhou, Y. (2016) Osteoinductive Effects of Free and Immobilized Bone Forming Peptide-1 on Human Adipose-Derived Stem Cells PLoS One. 11
5.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
6.) Zhang, X., Guo, J., Wu, G., & Zhou, Y. (2015). Effects of heterodimeric bone morphogenetic protein-2/7 on osteogenesis of human adipose-derived Stem Cells. Cell Proliferation, 48(6), 650–660. https://doi.org/10.1111/cpr.12218
7.) Lee HK, Finniss S, Cazacu S, Bucris E, Ziv-Av A, Xiang C, Bobbitt K, Rempel SA, Hasselbach L, Mikkelsen T, Slavin S, Brodie C. (2013) "Mesenchymal stem cells deliver synthetic microRNA mimics to glioma cells and glioma stem cells and inhibit their cell migration and self-renewal." Oncotarget. 4: 346-61.
8.) Robin TP, Smith A, McKinsey E, Reaves L, Jedlicka P, Ford HL. (2012) "EWS/FLI1 regulates EYA3 in Ewing sarcoma via modulation of miRNA-708, resulting in increased cell survival and chemoresistance." Mol Cancer Res. 10: 1098-108.
9.) Lu L, Song HF, Zhang WG, Liu XQ, Zhu Q, Cheng XL, Yang GJ, Li A, Xiao ZC. (2012) "Potential role of 20S proteasome in maintaining stem cell integrity of human bone marrow stromal cells in prolonged culture expansion." Biochem Biophys Res Commun. 422: 121-7.
10.) Tan F, Naciri M, Dowling D, Al-Rubeai M. (2012) "In vitro and in vivo bioactivity of CoBlast hydroxyapatite coating and the effect of impaction on its osteoconductivity." Biotechnol Adv. 30: 352-62.
11.) Dissanayaka WL, Zhu X, Zhang C, Jin L. (2011) "Characterization of dental pulp stem cells isolated from canine premolars." J Endod. 37: 1074-80.
12.) Liu S, Ginestier C, Ou SJ, Clouthier SG, Patel SH, Monville F, Korkaya H, Heath A, Dutcher J, Kleer CG, Jung Y, Dontu G, Taichman R, Wicha MS. (2011) "Breast cancer stem cells are regulated by mesenchymal stem cells through cytokine networks." Cancer Res. 71: 614-24.
13.) Liu Y, Xu X, Ma X, Martin-Rendon E, Watt S, Cui Z. (2010) "Cryopreservation of human bone marrow-derived mesenchymal stem cells with reduced dimethylsulfoxide and well-defined freezing solutions." Biotechnol Prog. 26: 1635-43.
14.) Medici D, Shore EM, Lounev VY, Kaplan FS, Kalluri R, Olsen BR. (2010) "Conversion of vascular endothelial cells into multipotent stem-like cells." Nat Med. 16: 1400-6.
15.) Li M, Jayandharan GR, Li B, Ling C, Ma W, Srivastava A, Zhong L. (2010) "High-efficiency transduction of fibroblasts and mesenchymal stem cells by tyrosine-mutant AAV2 vectors for their potential use in cellular therapy." Hum Gene Ther. 21: 1527-43.
16.) Li GC, Ye QH, Xue YH, Sun HJ, Zhou HJ, Ren N, Jia HL, Shi J, Wu JC, Dai C, Dong QZ, Qin LX. (2010) "Human mesenchymal stem cells inhibit metastasis of a hepatocellular carcinoma model using the MHCC97-H cell line." Cancer Sci. 101: 2546-53.
17.) Chadipiralla K, Yochim JM, Bahuleyan B, Huang CY, Garcia-Godoy F, Murray PE, Stelnicki EJ. (2010) "Osteogenic differentiation of stem cells derived from human periodontal ligaments and pulp of human exfoliated deciduous teeth." Cell Tissue Res. 340: 323-33.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 7530 | HUMSC | Human Umbilical Mesenchymal Stem Cells |
$812.00
|
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| MEDIA | |||||
| 7501 | MSCM | Mesenchymal Stem Cell Medium |
$139.00
As low as:
$90.00
|
||
| 7511 | MSCM-sf | Mesenchymal Stem Cell Medium-serum free |
$229.00
As low as:
$115.00
|
||
| 7521 | MSCM-acf | Mesenchymal Stem Cell Medium-animal component free |
$256.00
As low as:
$127.00
|
||
| 7551 | MCDM | Mesenchymal Stem Cell Chondrogenic Differentiation Medium |
$313.00
As low as:
$115.00
|
||
| 7531 | MODM | Mesenchymal Stem Cell Osteogenic Differentiation Medium |
$370.00
As low as:
$115.00
|
||
| 7541 | MADM | Mesenchymal Stem Cell Adipogenic Differentiation Medium |
$384.00
|
||
| 8298 | HMSC-A-qPCR | Human Mesenchymal Stem Cell Adipogenesis Detection qPCR Kit |
$311.00
|
||
| 8328 | HMSC-C-qPCR | Human Mesenchymal Stem Cell Chondrogenesis Detection qPCR Kit |
$311.00
|
||
| 8348 | SafraninO | Safranin O Staining Kit |
$117.00
|
||
| 8378 | ABlue | Alcian Blue Staining Kit |
$117.00
|
||
| 8678 | ARed-Q | Alizarin Red S Staining Quantification Assay |
$187.00
|
||
| MOLECULAR BIOLOGY | |||||
| 7509 | HMSC-bm gDNA | Human Mesenchymal Stem Cell-bone marrow Genomic DNA |
$438.00
|
||
| 7505 | HMSC-bm tRNA | Human Mesenchymal Stem Cell-bone marrow Total RNA |
$401.00
|
||
| 7507 | HMSC-bm miRNA | Human Mesenchymal Stem Cell-bone marrow MicroRNA |
$374.00
|
||
| 7506 | HMSC-bm Lysate | Human Mesenchymal Stem Cell-bone marrow Lysate |
$438.00
|
||
| 7504 | HMSC-bm cDNA | Human Mesenchymal Stem Cell-bone marrow cDNA |
$453.00
|
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