rat | ScienCell Research Laboratories

Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions to their partner neurons in the central nervous system (CNS), such as neuronal guidance during development, nutritional and metabolic support throughout life. Astrocytes have also been implicated in various pathological processes. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Much of what we have learned about astrocytes is from in vitro studies and astrocyte culture is a useful tool for exploring the diverse properties of this cell type.

RA-h from ScienCell Research Laboratories are isolated from CD® IGS rat hippocampus. RA-h are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RA-h are characterized by immunofluorescence with antibody specific to GFAP. RA-h are negative for mycoplasma, bacteria, yeast, and fungi. RA-h are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing RA-h in vitro.

Catalog No.	R1820
Country of Manufacture	United States
Product Code	RA-h
Size/Quantity	5 x 10^5 cells/vial
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping Info	Dry ice.
References	[1] Astrocytes, pharmacology and function. Edited by Sean Murphy. 1993 by Academic press, Inc. [2] Van der Laan, L. J. W., De Groot, C. J. A., Elices, M. J. and Dijkstran, C. D. (1997) Extracellular matrix proteins expressed by human adult astrocytes in vivo and in vitro: an astrocyte surface protein containing the CS1 domain contributes to binding of lymphoblasts. J. Neurosci. Res. 50:539-548. [3] Shao, Y. and McCarhy, K. D. (1994) Plasticity of astrocytes. Glia 11:147-155.