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- Rat Cardiac Fibroblasts
Cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes, are a source of paracrine growth factors, and are an important cellular component of the myocardial response to injury. CF are responsible for extracellular matrix synthesis in the heart during development and pathological conditions. Their proliferation and matrix synthesis is essential for scar formation at sites of myocardial infarction [1], cardiac fibrosis, and is often complicated by cardiac hypertrophy [2]. CF also respond to changes in microenvironments in pathological conditions by modulating integrin expression [3]. CF culture has been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.
RCF from ScienCell Research Laboratories are isolated from postnatal day 2 CD® IGS rat heart. RCF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RCF are characterized by fibroblast morphology. RCF are negative for mycoplasma, bacteria, yeast, and fungi. RCF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing RCF in vitro.
| Catalog No. | R6300 |
|---|---|
| Country of Manufacture | United States |
| Product Code | RCF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9. [2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54. ScienCell Research Laboratories TM [3] Burgess, M. L., Terracio, L, Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87. |
R6300No Publication available at this time
