C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

ScienCell’s Rat CRP Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Crp with a 96-well strip plate that is pre-coated with antibody specific for Crp. The detection antibody is a biotinylated antibody specific for Crp. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Crp with immunogen: Expression system for standard: NS0; Immunogen sequence: H20-S230. The kit is analytically validated with ready-to-use reagents.

Materials Required But Not Provided
1. Microplate reader.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multi-channel pipettes are recommended for large quantity of samples.
4. Clean tubes and Eppendorf tubes.

Assay type
Sandwich ELISA

Range
156 pg/ml- 10000 pg/ml

Sensitivity
< 10 pg/ml

Specificity
Natural and recombinant rat CRP

Cross-reactivity
This kit is for the detection of Rat CRP. No significant cross-reactivity or interference was observed. This claim is limited by existing techniques therefore cross-reactivity may exist with untested analogs.